1.Using Xanthine-Xanthine Oxidase system to generate superoxide ani-on, using H2O2 -Fe2 + system to generate hydroxyl radical, the role of scavenging oxygen free radical by Miltiorrhiza was studied by electron paramagnetic resonance and spin trapping.
用黄嘌呤——黄嘌呤氧化酶体系产生超氧阴离子,用H_2O_2—Fe~(2+)体系产生羟自由基,利用电子顺磁共振和自旋捕集技术,研究了丹参注射液的氧自由基清除作用。
2.Method Cultured CAEC were incubated with xanthine(26.3 μmol·L 1 )plus xanthine oxidase (17u·L 1 )(X XO)for 4 hours. The contents of NO,ET 1,PGE 2 and TXA 2 were measured by means of Griess assay,immunoradiation assay and ELISA,respectively.
方法培养的犬呼吸道上皮细胞与黄嘌呤（26．3μmol·L?1）及黄嘌呤氧化酶（17u·L?1）孵育4h后，应用酶联免疫、放射免疫及Gries还原法，测定条件培养液中NO，ET?1，PGE2和TXA2含量。
3.A study on the resistance of transfected cells with hSOD gene to superoxide a_nion_induced cytotoxicity caused by paraquat and xanthine (X)/xanthine oxidase (XO) is carried out in this paper.
研究了已获得稳定表达的hSOD基因导入细胞 (CMV -SOD细胞 )对百草枯 (paraquat)和黄嘌呤 (X) /黄嘌呤氧化酶 (XO)产生的超氧阴离子造成的细胞毒性的抵抗作用 .
4.The free radical amount of the cultured myocardial cells was measured by using electronic spin resonance (ESR)method. Rg_2 30μmol/L could inhibit the increase in free radical amount induced by xanthine 0.42 mmol/ L and xanthine-oxidase 5.3nmol/L.
用电子自旋共振法(ESR)测定了培养心肌细胞的自由基含量,Rg_230μmol/L 能显著抑制黄嘌吟0.42nmol/L、黄嘌呤氧化酶5.3nmol/L 所致的自由基含量的增多。
5.PEROXIDATION EFFECTS ON LIPOSOME OF ACTIVE OXYGEN SPECIES PRODUCED BY THE SYSTEM OF ADP-FeCl_3-XANTHINE-XANTHINE OXIDASE
ADP-FeCl_3-黄嘌呤-黄嘌呤氧化酶体系产生的活性氧对脂质体的过氧化影响
6.METHODS: Aortic smooth muscle cells from 4-6months-healthy abortive fetuses were incubated for 24 hours with xanthine (100 μmol/L) and xanthine oxidase (5 U/L) in vitro .
方法 :体外培养胎儿主动脉平滑肌细胞 ,加入含 10 0 μmol/L黄嘌呤 ,5U/L黄嘌呤氧化酶的无血清培养液 ,孵育 2 4h ,收集细胞上清液。
7.A novel technology to determine the activity of xanthine oxidase(XOD) through the chromogenic reaction of 4-aminoantipyrine(AAP),phenic acid(PA) and hydrogen peroxide(H_2O_2),which was produced via the oxidation of xanthine catalyzed by XOD,under the help of horseradish peroxidase(HRP) was(proposed).
研究了以辣根过氧化物酶-苯酚-4-氨基安替比林反应显色新体系,检测黄嘌呤氧化酶(XOD)活力的新方法。
8.The activity of SOD in hepatocytes and cell culture solution was determined by xanthine oxidase assay, and measured the git-up of GSH-Px and changes of H2O2 in cell culture solution.
用黄嘌呤氧化酶法检测对氨基苯胂酸对肝细胞内和细胞培养液中的SOD活性的影响，同时分别测定细胞中GSH-Px活性和细胞培养液中H_2O_2的变化；
9.The changes of morphology, ATP and malonaldehyde (MDA) contents, xanthine oxidase (XO) activity as well as the glutathione peroxidase (GSH-px) activity of rabbit aortic endothelial cells under hyperoxia (100% O_2) for 0-72 hours were studied.
本实验研究了离体兔主动脉血管内皮细胞在常压高氧(100%O_2)条件下(0～72h)细胞形态、三磷酸腺苷(ATP)、黄嘌呤氧化酶(XO)活性、丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GSH-px)活性的改变。
10.The levels of serum/plasma amylase,the changes of blood rheology, the contents of superoxide dismutase(SOD), xanthine oxidase(XO) and malondialdehyde(MDA) in pancreas and ileum and the pathology of pancreas and intestinal mucosa were examined.
观察各组血清酶学、血液流变学的改变 ,以及胰腺、回肠组织超氧化物歧化酶 (SOD)、黄嘌呤氧化酶 (XO)和丙二醛 (MDA)的含量 ,胰腺、肠粘膜的病理学形态改变。
