1.2. 1 PCR amplificatios were performed with universal bla_(IMP)、bla_(VIM)、bla_(SPM-1) and bla_(OprD2) primers,and the IMP、 VIM、 SPM-1 and OprD_2 encoding gene were amplified from the genome of 46Pseudomonas aeruginosa.
2．1 参照文献，设计合成IMP、VIM和SPM-1型获得性金属酶以及铜绿假单胞菌外膜蛋白OprD_2的上下游引物，以煮裂法提取细菌总DNA作为模板，运用PCR技术筛选含获得性金属酶基因和OprD_2基因的阳性菌株。
2.2. Using mechanical tests, FE-SEM, FE-TEM, AES, and XRD etc. analysis methods, the mechanical behavior and the GB chemical compositions of Fe-38Mn austenitic alloy prepared by VIM (vacuum induction melting) and VIM+ESR (electroslag remelting) were investigated.
2．采用低温力学性能测试以及场发射扫描电镜和透射电镜(配EDAX附件)、俄歇能谱分析、X射线衍射分析等手段，对真空熔炼(VIM)和真空熔炼后又经电渣重熔(ESR)的Fe-38Mn奥氏体钢的低温力学行为以及晶界成分分布进行研究。
3.The tumor was immunoreactive for EMA,CK7,Vim,also weakly immunoreactive for CgA,but not for CK34βE12,CD15,NSE,Syn.
免疫组化:EMA、CK7和Vim(+),CK34βE12、CD15(-),CgA弱(+),NSE和Syn(-)。
4.Method Twenty five cases of nodular fasciitis were studied with various immunohistochemical stains: Vim,Des,SMA,MyG,Mys,S100,NF, ⅤⅢ f,Lys and AAT.
②方法用波形蛋白（Vim）、结蛋白（Des）、平滑肌肌纤蛋白（SMA）、肌红蛋白（MyG）、肌球蛋白（Mys）、S100蛋白（S100）、神经原纤维（NF）、Ⅷ因子相关抗原（Ⅷf）、溶菌酶（Lys）和α1抗胰蛋白酶（AAT）进行免疫组化标记。
5.Bearing steels melted by 5 different processes, i.e. ESR, EBR, VAR, VIM-ESR and ESR-VAR_2are compared in order to improve the quality and the fatigue life of airborne bearings.
本文进行了ESR、EBR、VAR、VIM-ESR、ESR-VAR五种冶炼方法冶炼轴承钢的对比试验。
6.The conditions for the presence of bla_(IMP)-specific primers and bla_(VIM)-specific primers comprised 1 cycle of denaturation at 94℃ for 5 min, 30 cycles at 94℃ for 30 sec, 56℃ for 30 sec, and 72° C for 45 sec and then a final elongation at 72℃ for 7 min.
使用bla_(IMP)及bla_(VIM)引物时，PCR条件包括94°C×5分钟1个循环，94°C×30秒钟、56°C×30秒钟、72°C×45秒钟30个循环，最后72°C×7分钟延伸。
7.ESBLs,AmpC,VIM,IMP,OXA23,OXA-24 and outermembrane protein were detected. Reserpine synergistic inhibition test was used to study the active efflux mechanism.
检测ESBLs、AmpC酶、VIM、IMP、OXA-23和OXA-24产生及外膜蛋白表达情况,利血平协同抑制试验检测膜外排机制。
8.The stromal cells of SH were positive for CK L (5 cases), EMA (11 cases),Vim (10 cases),NSE (6 cases) and S 100 (3 cases), they had a few positive cells for Syn (2 cases),CEA (5 cases),p53 (1 case),CD34 (2 cases), while they were negative for CgA.
实性细胞 :阳性表达CKL5例、EMA 11例、Vim 10例、NSE 6例、S 10 0蛋白 3例 ,而Syn、CEA、p5 3、CD34分别有 2例、5例、1例、2例有少数阳性细胞 ,实性细胞不表达CgA。
9.METHODS β-Lactamase genes including TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,FOX,MOX and oprD2 were detected by PCR amplification in 33 PAE isolates.
方法对33株铜绿假单胞菌,采用PCR检测β-内酰胺类药物耐药相关基因(TEM、SHV、OXA-10群、PER、VEB、GES、CARB、IMP、VIM、SPM、GIM、DHA、FOX、MOX和OprD2)。
10.Brain-stems of 52 Wistar rats were stabbed with steel needle and the changes of GFAP (Glial Fibrillary Acidic Protein) positive astrocytes as well as VIM (Vimentin)positive astrocytes were demonstrated with LSAB immu noh istoc he mistry.
52只Wistar大白鼠针刺致脑干机械性损伤，LSAB法显示胶质原纤维酸性蛋白阳性（GFAP+）星形胶质细胞和波形蛋白阳性（VIM+）星形胶质细胞改变。
