1.Among 520 tobacco samples collected in Yunnan Province,71.74%,55.01% and 6.35% samples were infected by Tobacco mosaic virus(TMV),CMV and Potato virus Y(PVY),respectively.
在云南采集的520个花叶病样品中,烟草花叶病毒(Tobacco mosaic virus,TMV)、CMV和马铃薯Y病毒(Potato virus Y,PVY)总检出率分别为71.74%、55.01%和6.35%;
2.Tobacco Black Death Disease is one of the important tobacco diseases in Shandong Province.
烟草低头黑病(Tobacco Black Death Disease)是发生在山东烟区的主要病害之一。
3.Tobacco and capsicum were treated with 30μg/mL Harpin protein by spraying over the plant, and then Tobacco mosaic virus (TMV) was inoculated in the plants by mechanical inoculation after 5 days. The TMV concentration in leaves was determined by ELISA.
将烟草和辣椒用30μg/mL Harpin喷雾处理5d后,接种烟草花叶病毒(Tobacco mosaic virus,TMV),观察植株病症,并采用ELISA检测TMV含量。
4.Based on the coat protein (CP) gene sequence (480nt)of Tobacco mosaic virus (TMV), a 442bp fragment was amplified by reverse transcription polymerase chain reaction (RT-PCR) with the specific primers.
依据烟草花叶病毒(Tobacco mosaic virus，TMV)外壳蛋白(CP)基因序列(480nt)片段设计合成了一对引物，以病毒粗提RNA为模板，通过反转录—聚合链式反应(reverse transcription polymerase chain reaction，RT-PCR)扩增得到长度为442bp的目的片段。
5.From great to little followed : Nicotiana rustica L.> original cultivar> Oriental tobacco> Flue cured tobacco> Local air sun cured tobacco> Maryland tobacco> Burley tobacco Hearty shape leaf tobacco>.
种子千粒重排序为 :黄花烟 >原始种 >香料烟 >烤烟 >晒晾烟 >马里兰烟 >白肋烟 >心叶烟。
6.These contents in the flue-cured tobacco produced by Brazil was the highest, followed by Zimbabwe,Yunyan85,Zhongyan101,NC89 and K326.In four kinds of tobacco,the total contents of the 24 neutral fractions in(Oriental) tobacco was the highest,followed by Burly tobacco,Flue-cured tobacco and Maryland tobacco.
国内外不同品种的烤烟中中性香味成分的含量高低顺序为:巴西烤烟最高,其次是津巴布韦烤烟、云烟85、中烟101、NC89、K326; 4类烟草中中性香味成分含量最高的是香料烟,其次是白肋烟、烤烟、马里兰烟。
7.By Maillard Reaction,a series of important tobacco reaction flavour was developed with tobacco,such as Virginia tobacco and Buley tobacco.
根据梅拉德反应的本质与特性，以各种烟草如烤烟、白肋烟及四川糊毛晒烟等为原料，开发出一系列烟草反应物香料。
8.A study was carried out on the influence of condensate of oriental tobacco extract on chemical components and smoke components and flavor quality of flue-cured tobacco leaves during different concentration.The results showed that with the increasing of quality score of oriental tobacco extract condensate,the amount of reducing sugar in tobacco leaves decreased (by 1.49%？
研究了不同浓度条件下香料烟浸膏对烤烟烟叶化学成分、烟气成分和香吃味质量的影响.结果表明，随着香料烟浸膏质量分数的增大，烟叶中各种化学成分表现出不同程度的变化，其中还原糖下降幅度为1.49%？
9.12.26%).Further smoking quality test indicated that after the addition of condensate of oriental tobacco extract to flue-cured tobacco leaves,quality of aroma increased,while offensive odor and irritant decreased.Furthermore,with the increasing condensate of quality score in tobacco leaves,richness and sweetness of smoke improved in a way, and the flavor quality was the best when quality score (concentration) of oriental tobacco extract was 70%.
12.26%.进一步的评吸结果表明，在烤烟烟叶中加入香料烟浸膏后，可使香气质改善，香气量增加，杂气，刺激性减轻，而且当加入浸膏的质量分数增大时，烟气浓度和甜度有所增高，不同处理相比较，浸膏质量分数为70%时，烟气的香吃味质量最好.
10.In tobacco four genes have been cloned, NTETR1，NTERS1，NTHK1 and NTHK2.Screen tobacco cDNA library using two-component gene NTHK1 as probe and get its homologue gene NTHK2.The full-length of a two-component gene histidine kinase homologue NTHK2(Nicotiana tabacum histidine kinase) was isolated from tobacco.
另外，在烟草中也相继克隆了4个可能的乙烯受体基因:NTETR1，NTERS1，NTHK1和NTHK2。 本室克隆的两组分信号系统基因NTHK1为探针，筛选烟草cDNA文库，获得了一个同源基因NTHK2。
