1.The gene sequence for positive products showed that BDV P24 in goats from Chongqing was highly similar with that of H1766 strain detected from diseased horses(96. 51%)and also with that of the standard strain V and strain He/80(95. 35%). However, their amino acid sequences remained the same.
该 BDV P24片段核苷酸序列与马源BDV H1766株同源性最高,达96．51％,与标准株Strain V和 He／80同源性为95．35％,并且编码的氨基酸序列也相同。
2.E1 Tor Vibio Cholerae Strain SM_6 and Jiangsu 1d Strain
E1 Tor Vibio Cholerae Strain SM_6 and Jiangsu 1d Strain
3.The optimal growth near pH7.0. @According to its morphological and physiological qropertres, thrs methanogenie strain is considered to be a member of genus Methanobacterium, to which the name Methanobacterium thermoautotrophicum TH-6 strain is proposed for the time being.
根据形态和生理性状的研究,分离菌株暂定为嗜热自养甲烷杆菌TH-6菌株(Methanobacteriumthermoautotrophicum TH—6strain)。
4.PART ONE Investigation of BDV Natural Infection of Domestic Fowls and Ducks in Chongqing Objective:To investigate the natural infection of Borna Disease Virus (BDV) of domestic fowls and ducks in Chongqing and explore if the nucleotide sequence in BDV p24 was homophylic with the overseas strains, including standard Strain V and stain He/ 80 , detected from human and animals.
第一部分重庆地区家鸡和家鸭博尔纳病病毒自然感染状况研究目的:为了探讨重庆地区家鸡和家鸭博尔纳病病毒(BDV)的自然感染状况,分析重庆地区家鸡和家鸭自然感染的BDV p24核苷酸序列与国外人和动物来源BDV分离株及其标准株Strain V和He/ 80的同源性。
5.Tracing of the viral antigens by gold-labelled antibodies in the experimentally infected suckling mice with Chen strain hemorrhagic fever with renal syndrome virus and ultrastructural observation
Tracing of the viral antigens by gold－labelled antibodies in the experimentally infected suckling mice with Chen strain hemor
6.4 weeks after the last immunization, all the mice were challenged with H. pylori Sydney Strain 1 (1×107 CFU to each mouse) and sacrificed 4 weeks after the challenge. Their stomachs were collected for rapid urease test, histologic examination and quantitative culture to observe the gastric H. pylori densities.
免疫完成后4周以H． pylori Sydney Strain1菌株攻击小鼠1次（1×107 CFU／只），攻击后4周处死小鼠，取胃分别作快速尿素酶试验、病理检查及定量细菌培养，观察H．Pylori定植情况。
7.A New Study to Compensate Dynamic Measuring Error of Serial Multiple Degree of Freedom(DOF) Strain Gauge Force Sensor
A New Study to Compensate Dynamic Measuring Error of Serial Multiple Degree of Freedom（DOF） Strain Gauge Force Sensor
8.The gene sequence for positive product showed BDV p24 in the domestic fowls from Chongqing was homophylic with H1766 strain
结论:重庆地区家鸡中存在动物源性BDV自然感染,其感染的BDV p24核苷酸序列与动物来源的BDV H1766株及其标准株Strain V和He/ 80存在高度的同源性。 本研究不支持重庆地区家鸭中存在BDV自然感染。
9.Methods: the p24 fragment of BDV was detected by with fluorescence quantitative nested reverse transcriptase polymerase chain reaction (FQ-nRT-PCR) in peripheral blood mononuclear cells (PBMCs) from 120 domestic fowls and 120 ducks. And then the products of FQ-PCR from positive samples were cloned and sequenced before compared with those of the overseas strains, including Strain V and stain He/ 80, detected from human and animals.
方法:本研究采用荧光定量巢式逆转录酶聚合酶链反应(FQ-nRT-PCR)检测重庆地区120例家鸡和120例家鸭外周血单个核细胞(PBMCs)中BDV p24基因片段,并对阳性样本的荧光定量聚合酶链反应(FQ- PCR)产物进行基因序列测定,测序结果与国外人与动物来源的BDV分离株及其标准株Strain V和He/ 80进行序列比较。
10.The difference of the essential antigen coding gene of hog cholera field strains,Shimen strain and Chinese C strain had been studied. The results showed that hog cholera virus could been divided into two groups, six subgroups. Four field strains(02,03,06 and 07) and C strain, foreign C strain, Shimen strain, Japanese GPE strain, ALD strain, Italy Brescia strain were distributed into the same group, whereas the other two field strains and France Alfort strain were distributed into another group.
将猪瘟野毒株、石门系强毒株、兔化弱毒株及 1 982年分离的郑州野毒等毒株进行主要抗原编码基因差异研究 ,结果表明 :猪瘟病毒可分为 2个基因组 6个基因亚组 ,HCV- 0 2、0 3、0 6、0 7等 4个野毒株与国内的 C株、石门强毒株、国外的 C株、日本 GPE株、ALD株、意?
