1.Using this system, we detect the standard nucleic acid from America Acrometrix and China Clinical Laboratory Center to find its sensitivity, detect 10 neutralized test determined HBsAg positive specimen, 3 RIBA determined anti-HCV positive specimen and 3 Western Blot determined anti-HIV positive specimen to find its positive confirmatory rate with ELISA, detect clinical specimen to find its false-positive rate.
同时采用我中心酶免法(ELISA)初筛检结果阳性,经中和实验确认HBsAg阳性标本10份,经RIBA确认抗-HCV阳性标本3份,经WesternBlot确认抗-HIV阳性标本3份,检测该系统的阳性符合率; 检测大量临床ELISA筛检结果为阴性的标本来检测其特异性,并且每次实验设立内对照,阴阳性对照以进行临床标本检测。
2.[Methods] Content of HBV-DNA and HBVM in blood specimen and breast milk specimen of 167 HBV positive pregnant women and blood specimen of their 24 months old infants were delected by FQ-PCR and EIA.
方法应用荧光定量聚合酶链反应和酶免疫测定(EIA)技术对167例HBV携带孕妇血清、乳汁及婴儿24月龄血标本进行HBV-DNA定量和HBVM检测。
3.3) The PPARδ positive rate increases when the differentiation of the colorectal cancer specimen decreases, and they have statistic difference. ( P<0.05). The same is seen in the different enroding depth specimen and the different Dukes stage specimen(P<0.05).
在大肠癌标本中 :肿瘤的分化程度、肿瘤的侵犯深度和肿瘤的Dukes分期与PPARδ的表达阳性率有关 (P <0 .0 5 )。
4.Presents the investigation of the heat conduction behavior, internal thermal stress field of specimen under thermal shock and microscopic damage and failure of the tungsten based composites by combination of macroscopic and microscopic analyses and integration of material science and mechanics; describes a microscopic mechanical model based on the microstructure of components with macroscopic stress applied to the microscopic model as that of maximum principal stress at the center, and the fringe of the specimen, and the microscopic stress fields obtained through calculation by the finite element method, and concludes from test results that the maximum principal stress is first generated inside the test coupon,, and a failure will be first initiated in the inclusion if the material fails at this time; the maximum principal stress moves to the edge of the test coupon after the test coupon is heated for a period of time, and the failure will first be initiated in the base if the material fails at this time.
从材料设计的思想出发，采用宏观与微观、材料科学与力学相结合的方法，对钨基复合材料的热传导行为、材料在热冲击载荷下的内部热应力场及材料细观结构的破坏行为进行了详细的理论研究.根据钨基复合材料的细观组织结构建立了材料细观模型.在宏观分析的基础上，分析了材料微结构内部的破坏行为.分别取试件心部与边缘主应力值最大点的应力状态，施加在所建立的模型上，用有限元方法计算了模型内部的弹性应力场.结果表明：试件主应力最大值首先产生在试件内部，此时，如果材料发生破坏将先从夹杂中开始；加热一段时间后试件中的最大主应力值转移到试件边缘，此时材料发生破坏将先从基体中开始.
5.The paper mainly explores the effect of specimen size and aggregate diameter on concrete strength and guantitatively reveals the effect of the factors,such as specimen size, aggregate diameter, age and cement water ratio,etc, on strength by applying multivariate regression analysis.
本文主要探讨了二滩大坝混凝土试件尺寸和骨料粒径对强度的影响,并应用多元回归分析,定量地揭示了试件尺寸、骨料粒径、龄期和灰水比等诸因素对强度的影响。
6.Compared with NBT-BT6 specimen, the specimen added with proper amount of MnO shows a relative large increase of electromechanical coupling factor (kp) and significantly enhancedmechanical quality factor (Qm) accompanied by a relatively large decrease of piezoelectric constant (d33) and dielectric constant ( 33T/ 0) because of a "soft and hard" doping effect.
与NBT-BT6相比，添加适最MnO可以提高机电耦合系数k_p和机械品质因数Q_m，同时使介电常数ε_(33)~T／ε_0和压电常数d_(33)降低，表现出“软硬双性”添加物的作用。
7.Bergstrom (1973) thought that two pits of chephalic pos-terior lateral doublure in enrolled specimen of Arthrorhachis erraticus (Jackel)were the coaptative ones on the second pleural end and the bosses fromfront lateral pygidial doublure during enrollment. Hunt (1967) indicatedthat the lateral view of enrolled specimen in Arthrorhachis elspeth(Remond).
Bergstrom(1973)曾对Arthrorkachis的卷曲标本进行研究,认为头侧后腹边缘的两对凹坑为第二胸肋端和尾前侧腹边缘上的突起当卷曲时的接合坑,Hunt(1967)也曾列出Arthror-hachis elspeth(Remond)卷曲侧视标本。
8.〔Results〕 20 specimens from SARS probable cases in Wuhan were test by the one-step RT-PCR assay, specific DNA fragments as predicted were produced from specimen W20 by the assays using all 4 primer pairs, while one specific DNA fragment was produced from specimen W1 using primer pair BNIin-s/as.
〔结果〕利用该方法对武汉市非典型肺炎疑似病例进行SARS相关冠状病毒检测 ,其中样品W2 0用 4对引物检测均在相应的位置出现了DNA条带 ,样品W 1只有BNIin -s/as引物出现预期产物。
9.(1) we discuss Factors of genomic DNA degradation in Braconidae specimen. These factors include high temperature, oxidation and chemic modification. We should douse Braconidae specimen in 100% ethanol with 50mmol/L EDTA.
1．本文探讨了高温，氧化，化学修饰等影响茧蜂标本基因组DNA降解的因素，对室内保存寄生蜂标本提出应用含50mmol／L乙二胺四乙酸(EDTA)的无水乙醇固定，并在液面覆盖石蜡油等可行性建议。
10.By means of complince method the value of K_I (?) /P of a practical tapered DBC specimen was calibrated in the range of 0.20≤α/W≤0.75 and compared with the results calculated from no-sidegroove tapered DBC specimen by using the boundary collocation method.
试验用柔度的方法标定0.20≤α/w≤0.75范围内一种实用燕尾试样的K_I(BB_N)~(1/2)W~(1/2)/P 值,并与Srawley用边槽配置法对无边槽的燕尾试样计算出的K_IB(W)~(1/2)/P值作了比较。
