1.Methods Cultured rat VSMCs at passage 5 to 8 were used for experiments,S nitroso N acetypenicillamine(SNAP) was used as NO donor. Cells were preincubated for 24-48 hours in special culture dish with conditioned medium to reach quiescent,and then incubated in conditioned medium containing 0 5 mmol/L SNAP. After 8 to 10 hours,the cells were fixed and Bcl 2 expression was analyzed by ACAS 570 through fluorescent immunochemistry.
方法 以亚硝基乙酰青霉胺 (SNAP)作为 NO供体 ,将 5～ 8代的大鼠 VSMC接种于 Petri培养皿中 ,条件培养液培养 2 4～48h,换成含 0 .5 m mol/ L SNAP的条件培养液 ,8～ 10 h后 ,固定细胞 ,间接免疫荧光法检测抗原 ,借助粘附式细胞仪对细胞中 Bcl- 2蛋白表达进行定量检测。
2.2) TSN-incubation did not change the electrophoresis pattern and the amounts of SNAP-25, syntaxin and synaptobrevin/VAMP in rat cerebral synaptosomes, but made the synaptosomes completely resistant to BoNT/A-mediated cleavage of SNAP-25.3) TSN-incubation did not inhibit the directly cleavage of SNAP-25 on synaptosomal membrane fraction by the light chain of BoNT/A.
3)经川楝素处理的突触体膜组分不能抵抗A 型肉毒轻链对SNAP-25 蛋白的直接酶切作用; 4)川楝素能抑制A 型和C 型肉毒与突触体的结合,作用是浓度依赖的,升高反应温度或者用高钾刺激增强突触活动后抑制作用更明显。
3.The reliability and efficiency of the afore-mentioned methods are studied through tracing snap through and snap back responses of two cylindrical shells.
通过对二圆柱壳Snap through和Snap back响应的分析,研究了上述各种变步长增量迭代法的有效性和可靠性。
4.AccordingIy, IocaI infusion of SNAP-25 antisense into CA3 or CA1significantly reduced the long-term potentiation (LTP) of these regions,while simiIar treatment with SNAP-25 scrambIe or saline had no eff6ct onLTP.
相应地，在海马CA1和CA3区局部注射SNAP-25基因的antisense以后，海马CA1和CA3区的LTP显著降低，而注射scramble和saline则无此效应。
5.SNAP stimulated 5 fold increase of VEGF-A mRNA in COLO-357 cells and 4 fold increase of VEGF-A mRNA in CAPAN-1 cells.
而SNAP刺激细胞株COLO-357产生VEGF-AmRNA增加5倍,刺激细胞株CAPAN-1产生VEGF-AmRNA增加4倍。
7.In SMCs pretreated with CsA, absorbance of cells stimulated by PE decreased by 36.67%, but it could not be further altered by the additional treatment of SNAP, Sp-8-pCPT-cGMPS and Rp-8-pCPT-cGMPS.
VSMCs应用环胞素A预处理后,苯肾上腺素刺激诱发的VSMC吸光度降低36.67%,但是,上述吸光度不能被SNAP或Sp-8-pCPT-cGMPs进一步抑制或被Rp-8-pCPT-cGMPs升高。
8.METHODS SNAP or SNP or 8 Br cGMP was used for the treatment of ASMCs.
方法 人ASMCs用SNAP或SNP或 8 溴 环磷酸鸟苷 (8 Br cGMP)处理。
9.Our results show that the addition of SNAP and Sp-8-pCPT-cGMPS decrease absorbance of cells stimulated by phenylephrine (PE) by 27.32% and 36.57% respectively, whereas the addition of Rp-8-pCPT-cGMPS increases it by 79.02%.
结果显示SNAP和Sp-8-pCPT-cGMPs使苯肾上腺素刺激诱发的VSMCs吸光度分别降低27.32%和36.57%,而Rp-8-pCPT-cGMPs则可使苯肾上腺素刺激诱发的VSMC吸光度增加79.02%。
10.In SMCs pretreated with Ver, absorbance of cells stimulated by PE decreased by 22.05% and was further inhibited by the additional treatment of SNAP and Sp-8-pCPT-cGMPS, but was slightly promoted by the additional treatment of Rp-8-pCPT-cGMPS.
VSMCs应用维拉帕米预处理后,苯肾上腺素刺激诱发的VSMCs吸光度降低22.05%,并且上述吸光度可以被SNAP或Sp-8-pCPT-cGMPs进一步抑制,但可被Rp-8-pCPT-cGMPs轻微升高。
