1.96%(24/25) were positive of cps+/gdh+/epf+/mrp+/sly+/fbps+/orf2+ and only one virulent SS2 isolate was cps+/gdh+/epf-/mrp+/sly+/fbps+/orf2+.
结果显示:25株猪链球菌2型(SS2)国内分离株,96%(24/25)表现为cps+/gdh+/epf+/mrp+/sly+/fbps+/orf2+,仅1株表现为cps+/gdh+/epf-/mrp+/sly+/fbps+/orf2+;
2.Two strains of SS1 and SS7 were cps-/gdh+/epf-/mrp-/sly+/fbps-/orf2+,while,two strains of SS9 were cps-/gdh+/epf-/mrp-/sly-/fbps-/orf2+.
1株猪链球菌1型(SS1),表现为cps-/gdh+/epf-/mrp-/sly+/fbps-/orf2+; 1株猪链球菌7型(SS7)国内分离株,表现为cps-/gdh+/epf-/mrp-/sly+/fbps-/orf2+;
3.There were several genotypes and the strains of cps2/epf+/mrp+/sly+ were considered to be the most-virulent strains. The distribution of virulence-associate factors of China isolates was unknown.
结果显示：25株SS2国内分离株，96％(24／25)表现为cps2／gdh+／epf+／mrp+／sly+／fbps+／orf2+，仅1株表现为cps2／gdh+／epf-／mrp+／sly+／fbps+／orf2+。
4.Result of mouse virulence test showed that the genotypes of streptococcus,which were lethal to all of the mice,were mrp+,epf+ and sly+ serotype 2 strains. 1 serotype 2 strain and 1 serotype 7 strain could reproduce typical swine streptococcosis.
动物试验表明能100%致死小白鼠的猪链球菌基因型均为epf+mrp+sly+2型猪链球菌,1株2型和1株7型猪链球菌均能复制出典型的猪链球菌病例。
5.Results All PCR products were detected by electrophoresis on 1.2% agarose gels. With the 48 Streptococcus suis strains, the positive detection rates of cps2+ , mrp+ , epf+ , epf* + and sly+ were 16/48,14/48,12/48,3/48 and 26/48, respectively. The results were confirmed by bacteriological examination.
结果48株猪链球菌中,cps2检出率为33.3%(16/48)、mrp检出率为29.2%(14/48)、epf检出率为25%(12/48)、epf*检出率为6.3%(3/48)、sly检出率为54.2%(26/48),上述检测结果与菌株的毒力因子背景情况完全相符;
6.The recording unit of the SLY-31 is a 32k byte solid state memory composed of a single-Chip computer and CMOS RAMS. The current met- ar automatically measures the current,records the measured data with e sampling period required by the users.
SLY3-1型海流计使用单片机和静态 CMOS RAM 半导体存贮器组成8位32K字节的记录单元,它按使用者所要求的时间间隔,自动测量海流,记录测量数据。
7.MODEL SLY_(1-1) ACOUSTIC DOPPLER CURRENT METER
SLY_(1-1)型声学多普勒海流计
8.The positive detection rates of cps2/gdh+/epf+/mrp+/sly+/fbps+/orf2+ were 96% (24/25) and only one virulent SS2 isolate was cps2/gdh+/epf-/mrp+/sly+/fbps+/orf2+.
结合上述毒力因子分布情况，根据SS2的cps2J、mrp、orf2基因(virulent-associate sequence orf2)设计合成三对引物，建立三重PCR检测方法，并对PCR扩增产物进行测序、酶切、特异性及敏感性实验。
9.But sometimes Sly Boy can t run away from the dogs.
但有时，鬼小子却无法从那些狗身边跑掉。
