1.4. The primer was designed by AFLP P-GTG/M-CCA_(-31) marker ,and the marker named SCAR_(-282)transforms SCAR marker. Genetic distance was 10 cM.
4．根据AFLP标记P-GTG／M-CCA_(-313)克隆测序的结果设计引物，成功地将其转化成SCAR标记，遗传距离为10cM，命定名为SCAR_(-282)。
2.To improve the targeting of adenovirus vector for gene therapy,a fusion gene sCAR-EGF,in which epidermal growth factor gene was fused to the 3′ end of extracellular Coxsackie virus-adenovirus receptor gene,was constructed and cloned into shuttle plasmid pDC315 to obtain a recombinant plasmid pDC315-sCAR-EGF.
为了提高腺病毒载体用于基因治疗的靶向性,采用PCR和体外连接的方法构建了柯萨奇病毒-腺病毒受体(Coxsackievirus-AdenovirusReceptor)胞外段sCAR和表皮生长因子(Epidermalgrowthfactor)EGF融合基因,然后将此融合基因插入穿梭质粒pDC315。
3.The types of DNA molecular markers include RFLP, RAPD, DAF, SPAR, SSR, SCAR, STS, AFLP, CAPS, SNP, and so on.
DNA分子标记类型主要有RFLP、RAPD、DAF、SPAR、SSR、SCAR、STS、AFLP、CAPS、SNP等。
4.Methods: Human hypertrophic scar derived fibroblasts(HSF) were stably transfected with reconstructed plasmids named pU6-Rz182.G418 was applied to screen the cell clone steadily expressing ribozyme. The transfected cells were examined by MTT to draw the growth curve and evaluate cell growth.
方法:应用特异切割TIMP 1 的嵌合型核酶基因克隆pU6 Rz182 转染增生性瘢痕成纤维细胞(hypertrophic scar derived fibroblasts,HSF),G418筛选稳定表达核酶的细胞克隆,MTT法检测并绘制细胞生长曲线,观察核酶对细胞生长的影响;
5.A novel SCAR marker closely linked to the avirulence gene AVR-Pik~m in rice blast fungus Magnaporthe grisea
一个新的与稻瘟病菌无毒基因AVR-Pik~m紧密连锁的SCAR标记
6.RAPD marker S1338_(656) and AFLP marker P67M54_(172) were sequenced and successfully transferred into SCAR markers-SCOR_(204) and SCOR_(127) individually.
将筛选到的连锁RAPD标记S1338_(656)和AFLP标记P67M54_(172)成功转化为SCAR标记SCOR_(204)和SCOR_(127)。
7.Meeting of SCAR Specialist Groups,Aug,24-28,1987,Cambridge,England
南极研究科学委员会(SCAR)专家组会议
8.Development of a SCAR marker linked to avirulence gene AVR-Pik~m in rice blast fungus Magnaporthe grisea
一个与稻瘟病菌无毒基因AVR-Pik~m连锁的SCAR标记的分离
9.Analysis F_3 Individuals(BTAM428×ICS-12B) and Other Related Cultivars with SCAR
对高粱BTAM428×ICS-12B杂交F_3代及部分抗感品种的SCAR分析
10.Hypertrophic scar or keloid is common in clinic and the inception rate get higher and higher. It is not all clear for the internal cause and mechanism of scar formation. Most people go in harmony in present that scars are fibroblast cytosis and collagen fibers deposition in the scar tissue,but lack of dependable and valid treatment methods,and can not solve scar intrinsic problem of prevention and treatment.
增生性瘢痕或瘢痕疙瘩在临床上较常见,发病率越来越高,其产生的内因,形成的机理尚不完全清楚,目前比较一致的看法是瘢痕组织内成纤维细胞增多和胶原纤维沉积,但缺乏有效、可靠的治疗方法,未从根本上解决防治瘢痕的问题.
