1.Telmerase, a kind of reverse transcriptase, can use its own RNA template to synthesize the repeated sequences (5-TTAGGG-3)n of telomere DNA, and adds them to the chromosomes ends, maintains telomere length, results in cellular immortalization or oncogenesis. Telomerase was composed of six subunits: human telomerase RNA (hTR), telomerase associated protein (TP-1), human telomerase-reverse transcriptase (hTERT), heat shock protein (hsp90), molecular companion (p23) and dyskerin.
端粒酶由六部分组成:人端粒酶RNA(human telomerase RNA,hTR),端粒酶相关蛋白(telomerase-associated protein,TP-1),人端粒酶逆转录酶(human telomerase-reverse transcriptase,hTERT),热休克蛋白90(heat shock protein,hsp90),分子伴侣(p23)和全酶组装因子(dyskerin)。
2.Reverse engineering is a new discipline, which colligates modern design method、computer-Aided measurement、CAGD (computer-Aided Geometric Design ),CAD(Computer-Aided Design),CAM (Computer-Aided Made ). Material object reverse engineering of this thesis discussed refer to reconstruct the CAD modeling from the measured material object data, then use these models for analyses and manufacture of the products.
逆向工程是综合有仿制、现代设计方法、计算机辅助测量、CAGD(计算机辅助几何造型)、CAD(计算机辅助设计)、CAM(计算机辅助制造)等高科技在内的新兴学科，本文所讨论的实物反求工程 (reverse engineering)，是指从实物模型测得的数据，构造出该物体的CAD模型，继而将这些模型用于产品的分析和制造。
3.Methods The expression of BCSG1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) in surgical specimens from 93 cases of breast cancer patients selected randomly between September 1999 and May 2003 at Xinhua Hospital,Shanghai Jiaotong University School of Medicine. The correlation between BCSG1 gene expression and clinical parameters of breast cancer was analyzed.
方法随机抽取上海交通大学医学院附属新华医院1999年9月至2003年5月收治的93例女性乳腺癌病人的临床资料。 采用逆转录-链式聚合酶反应(reverse transcription-polymerase chain reaction,RT-PCR)检测标本BCSG1 mRNA表达,统计分析BC-SG1基因表达与乳腺癌各项临床病理参数的相关性;
4.Methods Immunohistochemical assay and reverse transcriptase polymerase chain reaction(RT-PCR) were used respectively to detect HCV antigens(NS3, NSS and CP10) and HCV RNA positive-and negative-strand in kidney, heart, pancreas, and intestine from 9 hepatitis C patients.
方法分别采用免疫组织化学和逆转录多聚酶链反应（reverse transcriptase polymerase chain reaction， RT－PCR）方法检测9例丙型肝炎肾、心、胰腺和肠等肝外组织HCV多种抗原和正链、负链HCV RNA。
5.The rapid diagnosis technique based on the reverse transcription polymerase chain reaction (RT-PCR), the isolation and identification of field viruses and the sequencing and cloning of S1 gene, and epizootiology of ARV infections in Guangxi were studied in the thesis.
课题分别对ARV的反转录-聚合酶链式反应(reverse transcription polymerase chain reaction，RT-PCR)快速诊断方法、病毒的分离和鉴定、流行病学的调查、以及广西地方分离株S1基因的核苷酸序列等进行了研究。
6.In our lab , By mRNA DDRT-PCR (mRNA differential display reverse transcription-PCR ) and RACE (Rapid amplification of cDNA ends ) , the STS gene and ALDH gene of Chinese wild grape was cloned and separated from Vitis pseudoreticulata Baihe-35-1 , which has a high resistance to Uncinula necator schw. Burr.
本实验室研究人员通过mRNA差异显示技术(mRNA differential display reverse transcription-PCR , DDRT-PCR)及cDNA 末端快速扩增技术(Rapid amplification of cDNA ends, RACE),从高抗白粉病的华东葡萄白河-35-1(Vitis pseudoreticulata)中克隆分离得到中国野生葡萄STS 基因、ALDH 基因。
7.Using a reverse transcriptase polymerase chain reaction (RT-PCR) the 540bp-long S1 gene of two strains of avian Reovirus (ARV) (S1133,1733) were amplified successfully.
利用RT-PCR(Reverse Transcriptase-Polymerase chain Reaction)技术,扩增出禽呼肠孤病毒S1133、1733两毒株长为540bp的S1基因片段。
8.Methods MCL-1 AS-ODN was transfected into cultured melanoma cell line WM451 with lipofectamine 2000. The expression of Mcl-1 nucleotide and protein was detected by Reverse transcription polymerase chain reaction (RT-PCR)、Western blot and Immunocytochemistry, and apoptosis was assayed by Electron microscope and FAC.
方法:培养恶性黑素瘤细胞WM451,在阳离子脂质体的辅助下转染MCL-1的反义寡核苷酸利用逆转录聚合酶链式反应(Reverse transcription polymerase chain reaction,RT-PCR)的方法观察转染前后MCL-1基因表达情况; 免疫组化及Western Blot法检测肿瘤细胞转染前后MCL-1的蛋白表达情况。
9.Methods: Mainly with methods of in situ reverse transcription polymerase chain reaction (In situ RT-PCR).
方法 以原位逆转录聚合酶链式反应(In situ reverse transcription polymerase chain reaction,In situ RT-PCR)为主要研究方法。
10.the expression of transforming growth factor-β_1 (TGF-β_1) . type I TGF-β receptor(T β RI). type Ⅱ TGF-β receptor (T β RⅡ)and fibronectin mRNA in renal cortices of all three groups were measured by quantitative reverse transcription polymerase chain reaction(RT-PCR).
观察8周后，用半定量逆转录多聚酶链式反应(reverse transcript-polymerase chain reaction,RT-PCR)检测各组肾皮质Ⅰ型转移生长因子-β受体(type Ⅰ transforming growth factor β receptor，TβRI)、Ⅱ型转移生长因子-β受体(TβRⅡ)和纤维连接蛋白(fibronectin．FN)mRNA的表达；
