1.Methods ELiSA,enzyme immunoassay using Swiss production workstations MRP150 weremeasured,the results were statistically analyzed,results PreS_1Ag HBeAg positive rate was higher than the results,PreS_1Ag closely associated withviral replication in HBeAg indicators,But both used to judge thereplication of hepatitis B virus activity and do not match exactly thesame value. PreS_1Ag more sensitive than HBeAg,the representative of a widerrange.
方法采用酶免法,用瑞士产酶免工作站MRp150进行检测,并对检测结果进行统计分析。 结果PreS1Ag阳性率高于HBeAg阳性率,PreS1Ag与病毒复制指标HBeAg密切相关,但两者用来判断乙肝病毒的活动性和复制的应用价值并不相完全等同,Pres1Ag比HBeAg更敏感,代表的范围更广。
2.The sera of 202 patients with different types of HBV infection were assayed for PreS2 proteins(PreS2)and Anti-PreS2 antibody (anti-PreS2) by ELISA.
本文用酶联免疫法(ELISA)对202例各类乙型肝炎病毒(HBV)感染者血清前S2蛋白(PreS2)和前S2抗体(抗PreS2)进行了检测。
3.The similar results also occurred in using Dane parti-cle and gene expression PreS1 protein as antigens to detect the anti-PreS 1 antibody in PreS_1 syntheticpeptide immune serum.
用Dane颗粒和基因表达PreS＿1蛋白为抗原也可以检测到PreS＿1合成肽免疫血清中的PreS＿1抗体。
4.Methods HBV proS1 gene was amplified by polymerase chain reaction(PCR),HBV preS1 bait plasmid,named pAS2-1-preS1,was constructed by yeast two hybrid system 3 and verified by auto-sequencing assay,pAS2-1-preS1 was transformed into the yeast AH109 and preS1-BD fusion protein expressed in the yeast cells was confirmed by Western blotting method.
方法PCR扩增HBV前S1基因序列,根据酵母双杂合体系构建饵质粒pAS2-1- preS1,并测定序列; 饵质粒转化入酵母菌AH109,Western印迹法证实前S1-BD融合蛋白在酵母细胞中的正确表达。
5.The complate nucleotide sequences of the cloned preS genes(preS-HZ and preS-LZ,GenBank accession numbers:Af325674 and AF325675 respectively) were 522bp long,encoding 174aa.
克隆的HBVpreS基因杭州分离物 (preS HZ)和兰州分离物 (preS LZ)全长 5 2 2个核苷酸 ,编码174个氨基酸。
6.L protein includes gene preSl, preS2 and S, M protein includes gene preS2 and S, S protein is coded by gene S. The functions of M protein are not clear.
preS2-S基因表达M蛋白，包含preS2和S。 目前，对中蛋白在乙型肝炎病毒生活周期中的功能及与其它乙型肝炎病毒蛋白间的相互作用尚不清楚。
7.Study of Correlation Between Hepatitis B Virus PreS_1 Protein (PreS_1) and HBV-DNA with Hepatitis B virus Markers (HBV M)
PreS_1和HBV-DNA与HBV-M相关性分析
8.Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method.
方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
9.Conclusion: The imaging findings of PRES are typical, so MRI should be the first choice in its diagnosis.
结论:PRES的MRI表现具有特征性,MRI应作为诊断本病的首选手段。
10.The cAb well reacted with preS1 in HBV eAg positive serum.
嵌合抗体可以很好的与病毒preS1结合,对阳性血清的检测效果与鼠单抗一致。
