1.The microarray experiment results were further confirmed by Quantitative Real-Time PCR (QRT-PCR). We identified 5 genes whose expressions changed more than 2-fold between RSLV mutant and WT leaves. They encode phloem protein 2 (PP2), ferritin, pectin methyl esterase (PME), chlorophyll a/b binding protein (CAB protein) and pyruvate decarboxylase (PDC), respectively.
进一步采用实时定量PCR技术验证芯片实验结果,最终确定所检测的基因中,有5个基因在突变型和野生型间表达差异显著,分别是韧皮部蛋白2(phloem protein2,PP2)、铁蛋白(ferritin)、果胶甲酯酶(pectin methyl esterases,PMEs),以及叶绿素a/b结合蛋白和丙酮酸脱羧酶等。
2.Granular form of vegetative storage proteins was observed in the large central vacuoles of the secondary phloem parenchyma cells and secondary phloem ray parenchyma cells in the samples fixed in 4% glutaraldehyde.
在用戊二醛固定的标本中,营养贮藏蛋白质呈颗粒状,积累在中央大液泡里。
3.The secondary phloem parenchyma cells and secondary phloem ray parenchyma cells were the preferential sites for protein storage to the xylem parenchyma cells whereas a large amount of vegetative storage proteins was present in xylem, especially in the primary xylem and the inner secondary xylem of the extended stems.
营养贮藏蛋白质优先在次生韧皮部的韧皮薄壁细胞和韧皮射线薄壁细胞中积累,在新梢伸长生长停止后,新梢基部茎的木质部中也积累了相当数量的营养贮藏蛋白质,主要分布在初生木质部和内侧次生木质部的各种生活的薄壁细胞中。
4.Results showed that the reducing sugar content in phloem was much higher than that in xylem, and a significant difference was found in the phloem of different poplar strains.
结果表明:各杨树品系木质部的还原糖含量远远低于韧皮部的含量,且韧皮部的还原糖含量差异显著。
5.Contents and composition of amino acids in grain, liquid in endosperm cavity ,phloem exudate from peduncle, flag leaf, phloem exudate from flag leaf and xylem sap from roots were measured during grain development, using Triticum aestivum L. cv. Jingdong 6. The main amino acids were Gln+Glu,Pro,Ala,Asn+Asp,Ser and Gly in grain.
以京冬 6号为材料分析了小麦籽粒发育期籽粒、籽粒腹腔液、穗梗韧皮部汁液、旗叶、旗叶韧皮部汁液和根系伤流液中氨基酸含量及组分的变化。 籽粒中以谷氨酰胺和谷氨酸 ( Gln+Glu)、脯氨酸 ( Pro)、丙氨酸( Ala)、天冬氨酸和天冬酰胺 ( Asn+Asp)、丝氨酸 ( Ser)和甘氨酸 ( Gly)为主。
6.We have observed the following characteristics of anatomical structure and development: (1) The primary roots belong to archetype and the lateral roots occur in the position toward the xylem of the vascular cylinder; (2) The root endodermis consists of thicken casparian strips, and there are from two lo four layers parenchyma cells near the pericycle; (3) the pith and resins canals are absent in the toots; (4) Secondary structure occurs in the root hair zone, and vascular cambium is formed from the parenchyma cells between the primary xylem and primary phloem; (5) Tanning materials are abundant in pericycle cells and phelloderm cells; (6) Sclereids are found in secondary phloem, and rays are rather rich in secondary xylem.
结果表明:气生不定根具有以下结构特征和发育特点:(1)根为二原型,侧根正对着木质部发生;(2)幼根内皮层细胞具凯氏带加厚,维管柱内紧挨中柱鞘内侧具有2~4层大型薄壁细胞;(2)根中无髓和树脂道;(4)次生结构发生于根毛区,维管形成层由初生木质部和初生韧皮部之间的薄壁细胞转化形成;(5)中柱鞘和栓内层细胞富含单宁类物质;(6)次生韧皮部有石细胞,次生木质部射线发达。
7.Primary phloem PHLOEM derived from the procambium in the young plant.
初生韧皮部:指幼嫩植物中来源于原形成层的韧皮部。
8.The result showed that Cu, Zn, Fe, Mn mostly distributing in the cortex, secondly distributing in the xylem or the marrow, the content is lower in the phloem of rhizome tissue on the cultivated Rheum.
结果表明,种植大黄根茎中铜、锌、铁、锰等元素主要分布在皮层,其次是木质部或髓部,元素含量较低的是韧皮部。
9.Endomitosis occurs regularly in certain tissues, such as the phloem cells of some leguminous plants.
核内有丝分裂通常发生在特定组织中,如一些豆科植物的韧皮部细胞。
10.THE EFFECT OF ACETYCHOLINE, LatA AND FAA ON PHLOEM ASSIMILATES TRANSLOCATION OF Raphanus sativus L.
乙酰胆碱、LatA和FAA对萝卜韧皮部同化物运输的影响