2.To further demonstrate the interaction between GR and JAB1 and the effect of JAB1 on GR, PCR was performed to amplify GR LBD fragments and it was cloned into the bait vector pGBKT7 to create the plasmid pGBKT7 GR LBD. The plasmid was used as bait to screen a cDNA library constructed in the pACT2 vector. Transformants were plated on SD/-Ade/-His/-Leu/-Trp.
构建与Gal4 BD融合表达的载体pGBKT7 GRLBD ,与构建于pACT2载体上的人骨髓cDNA文库杂交 ,在SD/ Ade/ His/ Leu/ Trp选择培养板上培养 ,经X α gal检测 ,阳性克隆片段插入pGEM TVector载体 ,测序 ,再经酵母双杂交和GSTpulldown蛋白质结合实验验证其结合作用 ,应用反映GR转录活性的CAT报告基因检测JAB1对GR的调节活性 .
3.APPLICATION OF FACULTATIVE ANAEROBIC-PACT TO TREATMENT OF CHEMICAL FIBER TAIL WASTEWATER
兼氧-PACT工艺处理化纤废水尾水
4.Results The cotransfection of pAct.
结果 转染糜蛋白酶基因双链RNA和 pAct .
5.With aerobic bacteria H12, the CODcr removal rate of fumaric acid-containing organic wastewater reached 76.4%, that of camphor-containing organic wastewater reached 76. 5% . Using the method of PACT, the CODcr removal rate of Fumaric acid-containing organic wastewater reached 86% with the bacteria TH.
好氧菌H12菌处理时，富马酸废水COD去除率达到76.4％，樟脑废水COD去除率达到76.5％，TH的PACT法处理富马酸废水，COD去除率达到86％。
6.PACT (Portable Application Code Toolkit) is a comprehensive, integrated, and portable software development environment created for applications having unique requirements not met with available software.
PACT是一个综合的，集中的，可移植的软件开发环境，它对应用软件的创造有独特的要求，还没有被目前软件满足。
7.We relied in the Middle East on the Baghdad Pact, and yet when the Iraqi government was changed, the Baghdad Pact broke down.
在中东，我们依赖有《巴格达协定》，但伊拉克政府变更后，撕毁了《巴格达协定》。
8.First comes the legislation of the international organizations, such as the UN Drug Banning Pact, European Anti-Money Laundering Pact, The Instruction About Preventing Criminals From Using Financial System To Launder Money issued by EC (European Community) and the anti-money laundering regulations of the American countries.
首先论述了国际组织的反洗钱立法，如联合国禁毒公约、欧洲反洗钱公约、欧洲共同体《关于防止犯罪分子使用金融系统洗钱的指令》和美洲国家组织反洗钱规则等，接着论述了有关国家和地区反洗钱的立法，如美国、德国、瑞士、台湾地区和香港地区的反洗钱立法规定。
9.Pact Ⅱ: The synthesis of the side chain of vitamin E based on the utilization of 4(R)-methyl-tetrahydropyran-2-one.
2、4R-甲基戊酸内酯在维生素E合成中的应用；
10.an organization of people (or countries) involved in a pact or treaty.
以公约或条约维系的民族或国家的组织。
