1.At the 1,7,14,21 days after exposed,8 rats of each group were used to evaluate expressions of Notch2,Notch4 in lungs by immunohistochemistry and the level of Nothch2,Notch4 mRNA by reverse transcription polymerase chain reaction(RT-PCR).
于生后1、7、14、21d留取肺组织标本,免疫组织化学法检测Notch2、Notch4的表达,RT-PCR检测Notch2、Notch4 mRNA的表达。
2.RT-PCR and Western-blot were employed to assay the expression of Notch pathway signal molecules: Notch1, PS1, RBP-Jk and HES1. The results revealed that the percentage of neurons in BMP-2 group was much higher as compared with EGF and Ctrl (control) groups;
用RTPCR和Westernblot检测Notch信号分子Notch1、PS1、RBPJk和HES1的表达水平。 结果表明:BMP2组分化为神经元的比例明显高于对照(control,Ctrl)组和EGF组;
3.Conclusion Notch-1 may down-regulate the differentiation of RPC, and the inhibition of Notch-1 may promote RPC differentiating into RGC.
结论Notch-1对RPC的分化具有负向调控作用,阻断Notch-1能促进RPC向RGC分化。
4.METHODS: SD rats were randomly divided into 4 groups: model group(group A)、 moderate-dose Qinggan Huayu Formula group(group B)、high-dose Qinggan Huayu Formula(group C)、normal control group(group D),and to detect the expression of γGT、Notch1、Delta、 NF-κB by enzyme-histochemisty and immunohistochemistry methods.
方法:SD大鼠随机分为4组:模型组(A组)、清肝化瘀方药等效剂量组(B组)、清肝化瘀方药大剂量组(C组)、对照组(D组),分别用酶组化及免疫组化方法检测γ-谷胺酰转肽酶(γ-GT酶)、Notch1、Notch1配体Delta、NF-κB的表达变化。
5.Results The expression of Notch 1 was initiated in early bell stage in inner-enamal epithelium pre-odontoblasts and dental papilla mesenchymal cells.
结果Notch 1的表达始于钟状中期。 在钟状中晚期,Notch 1的表达在牙乳头间充质、成牙本质细胞层呈弱阳性,在内釉细胞层呈阳性。
6.The Expression of Notch1 in Prostate Cancer and Its Effects on the Biological Behavior of the Prostate Cancer
Notch1在前列腺癌组织中的表达以及外源性 Notch1对前列腺癌细胞的生物学行为的影响
7.Regulation of the Key Transcription Factor RBP-J of Notch Signaling Pathway by PcG Proteins HPC2 and RING1
PcG蛋白HPC2和RING1对Notch信号途径关键转录因子RBP-J的调控作用的研究
8.CONCLUSIONS: Notch signal pathway might involved in the maturation of dental pulp cells, and DEX at the concentration of 10-7M could depress the expression of DSPP and increase that of Heyl.
结论:Notch 信号途径可能参与了牙髓细胞的诱导分化过程,10-4M浓度DEX能抑制DSPP的表达,促进Hey1的表达。
9.“Mutations in NOTCH 1 cause an early developmental defect in the aortic valve,” said Dr Vidu Garg, an assistant professor of paediatrics and molecular biology.
「NOTCH 1基因突变，引起主动脉瓣膜的早期发育缺陷，」小儿科和分子生物学助理教授维杜．加尔格博士表示。
10.Methods RPC of 14-day embryonic SD rats were induced and differentiated in the culture medium with Notch-1 antisense oligonucleotides (experimental group) or missense oligonucleotides (control group) for 14 days. The condition of growth and differentiation of the cells were observed daily under the phase-contrast microscope. RGC were identified by Thy1.1 immunocytochemistry methods and the cellular number was counted.
方法分离培养胚胎14d龄Sprague-Dawley大鼠的RPC,实验组和对照组分别用含有Notch-1反义寡核苷酸链和无关序列寡核苷酸链的培养液进行诱导分化14d,倒置相差显微镜每天观察细胞的生长和分化情况,Thy1.1标记RGC并进行计数。
