1.The results showed there was a same mutation in BMPR-IB gene (A746G)of China Merino fecundity sheep and Hu-Yang. There were three genotypes (BB, B+, ++), the BB and B+ mutation genotype was superior in prolific China merino fecundity sheep and HU-YANG.
实验结果表明:中国美利奴多胎群体和湖羊在BMPR-IB基因的相应位置上发生了与Booroola Merino羊相同的突变位点(A746G),在这两个品种(系)中出现了3种基因型(BB、B+、++),该基因的BB和B+基因型在中国美利奴多胎品系和湖羊群体内为优势基因型。
2.15 Borderdale,18 Poll Dorset and 15 German Mutton Merino were treated with progesterone sponges plus PMSG and FSH;
CIDR、PMSG、FSH联合使用对初产供体羊Borderdale(波德代)3♂15♀、Poll Dorset(无角陶赛特)5♂18♀和Ger-man Mutton Merino(德国肉毛兼用美利奴—德美)3♂15♀进行同期发情和超数排卵。
3.In China,mtDNA haplotype diversity was the highest in Qinghai Tibetan sheep,followed then by Gansu Tibetan sheep,Gansu Alpine Merino,Qinghai Merino,Gannan Tibetan sheep,Small-tailed Han sheep,Tan sheep,Hu sheep and Minxian Black Fur sheep.
中国绵羊群体的单倍型多样度在青海藏羊、甘肃藏羊、甘肃高山细毛羊、青海细毛羊、甘南藏羊、小尾寒羊和滩羊群体中较高,但在湖羊和岷县黑裘皮羊中较低;
4.The Suffolk and Dorset sheep were first grouped together with the nearest genetic distance of 0.345 25,and then together with Charolais,finally together with Chinese Merino. The genetic distance between Chinese Merino and Charolais was the farest of 0.516 39.The results could be useful as an initial guide in evaluating the level of genetic variation,developing conservation strategies and predicting heterosis.
4个肉用绵羊品种间的遗传距离相对较远,萨福克羊和陶赛特羊先聚在一起,他们之间的遗传距离最近,为0.345 25,然后与夏洛来羊聚在一起,最后与中国美利奴羊聚在一起,中国美利奴羊与夏洛来羊之间的遗传距离最远,为0.516 39。 研究结果对我国肉用绵羊资源的评估、保存和预测杂种优势具有一定的指导意义。
5.The lambs of Staffropol Fine-wool sheep derived from Australian Merino and New Zealand Merino slieep (Australian blood accounted for 37.78%) began to appear highcrimped abdominal fibre (HCAF) (10.47%) at 3 month of age (70～90 days).
经澳大利亚和新西兰美利奴羊导血的我国镇南种羊场斯达夫细毛羊(澳血含量为37.78％)所产的羔羊于3月龄内(70～90天)开始出现高弯腹毛(约占10.47％)。 首先出现在脐前部和腹缘左右内侧部,以靠近左右腹侧线内的腹前部和腹中部出现得最多。
6.Meanwhile Ep was polymorphism in Small tailed Han sheep, Qinghai Merino, Tibetan sheep in Qinghai, Tibetan sheep in Gasu and wasn’t polymorphisms in Gansu Alpine Merino and Tan sheep.
而Ep在小尾寒羊、青海细毛羊、青海藏羊、甘肃藏羊中存在多态性，在甘肃高山细毛羊、滩羊中不存在多态性；
7.Polymorphism of mutation in FecGH of GDF9 gene and mutation in FecX B and FecXG of BMP15 gene were studied by forced PCR RFLP method as candidates fo r prolific genes in 7 breeds or strains of sheep such as Hu,Chinese Merino meat Prolificacy strain,Suffolk,Dorset,Charolais,China Merino and Romney Hills.
以绵羊GDF9基因FecGH突变和BMP15基因FecXB和FecXG突变为候选基因,采用PCR-RFLP方法研究其在湖羊、夏洛来、陶赛特、萨福克、中国美利奴肉用多胎品系、中国美利奴羊和罗米丽羊7个品种中的多态性.
8.The sequencing results showed that there was a mutation (T→C) at 877 bp of exon 1 of INHA gene in Small Tail Han sheep and Corriedale sheep, the same mutation did not exist in Chinese Merino sheep and South African Mutton Merino sheep.
小尾寒羊、考力代绵羊在INHA基因外显子1中发生了1处碱基突变(877T→C),中国美利奴绵羊、南非肉用美利奴绵羊没有发生这种突变;
9.For primer 2, three genotypes (AA, AB and BB) were detected in four sheep breeds. In Small Tail Han sheep, Chinese Merino sheep, Corriedale sheep and South African Mutton Merino sheep, frequency of AA genotype was 0.017, 0.216, 0.115, 0.429, frequency of AB genotype was 0.102, 0.317, 0.269, 0.500, frequency of BB genotype was 0.881, 0.467, 0.616, 0.071, respectively.
统计结果表明:小尾寒羊、中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊AA基因型频率分别为0.017、0.216、0.115、0.429,AB基因型频率分别为0.102、0.317、0.269、0.500,BB基因型频率分别为0.881、0.467、0.616、0.071;
10.AMY was monomorphic in Small tailed Han sheep, Tan sheep, Gansu Alpine Merino and was polymorphisms in Qinghai Merino, Tibetan sheep in Gasu, Tibetan sheep in Qinghai.
AMY在小尾寒羊、甘肃高山细毛羊、滩羊中呈单态，在青海细毛羊、青海藏羊、甘肃藏羊中存在多态性；
