1.Studies on Immunotherapy against Melanoma in Mice Using IL2 Gene-Transfected B16 Melanoma Vaccine Combined with Specific CTL Cells
Studies on Immunotherapy against Melanoma in MiceUsing IL2 Gene－Transfected B16 Melanoma VaccineCombined with Specific CTL Ce
2.Objective: Basal cell carcinoma(BCC), squamous cell carcinoma (SCC), Bowen’s disease(BOD),actinic keratosis(AK)and seborrheic keratosis (SK)are the commonest non-melanoma skin cancers (NMSC ). BCC and SCC comprise the majority of NMSC. In the recent years, the incidence of BCC and SCC continue to rise.
目的: 基底细胞癌(basal cell carcinoma, BCC)、鳞状细胞癌(squamous cell carcinoma, SCC)、Bowen 病(Bowen’s disease,BOD)、光线性角化病(actinic keratosis, AK)及脂溢性角化病(seborrheic keratosis, SK)是最常见的非黑素瘤皮肤肿瘤(non-melanoma skin cancers,NMSC)。
3.Briefly,tumor cells were diffusely positive for the Melan-A,Melanoma(Pan),Vimentin and Actin,focal expression of HMB-45.In contrast,S-100 protein and Syn were absent.
肿瘤细胞Melan-A及Melanoma(Pan)强阳性,HMB45灶性强阳性,Vimentin及Actin阳性,S-100、Syn阴性。
4.We have verified by using cultured cell on in vitro test, that when we add 3% of Fissione-Y to mouse melanoma cell line, B16, we can inhibit 63.8% of melanin formation.
通过培养细胞的In vitro试验、在白鼠melanoma细胞B16添加3％的Fissione-Y,观察到具有抑制63．8％黑色素的效果。
5.Methods: B16F10 melanoma cells were inoculated,sc,iv and ip respectively into mice to establish 3 melanoma and lung metastasis models. Effect of DTIC,an anti-melanoma drug,on the 3 animal models was investigated.
方法:分别通过皮下、静脉和腹腔注射方式,将B16F10黑素瘤细胞接种于小鼠体内,建立3种黑素瘤及其肺转移小鼠模型,并以达卡巴嗪作为试药,考察其对这3种模型小鼠的作用。
6.Studies on the Immunotherapy Against Melanoma in Mice by Using IL2 Gene-Transfected B_(16) Melanoma Vaccine Combined with Specific CTL Cells
用IL-2转基因B_（16）黑色素瘤瘤苗与特异性CTL细胞进行小鼠肿瘤免疫治疗的研究
7.Results Proliferation of B16 melanoma cells in vitro was significantly was inhibited by ursolic acid phospholipids nanoparticles in a time-and dose-dependent manner. After B16 melanoma cells were treated with UA-PL-NP for 24 h, the IC50 value was 7. 68 mg/L. The IC50 value of C-DDP was 9. 33 mg/L for 24 h.
结果MTT检测熊果酸纳米脂质体微粒对B16细胞增殖有明显抑制作用,并呈浓度和时间依赖性,UA-PL-NP作用24hIC50值为7．68 mg／L,顺铂作用24 h IC50值为9．33 mg／L;
8.Results： Following immunization with HAC60, HAC75 and HAC97 obtained through affinity chromatography, the incidence of melanoma in the mice received B16 melanoma implantation decreased, the time of tumor development delayed and tumor weight reduced.
结果：亲和层析法获得的HAC60、HAC75和HAC97免疫小鼠后，小鼠移植肿瘤的发生率降低，肿瘤平均出现时间延迟，平均肿瘤重量明显减轻；
9.Objective:To evaluate the chemotherapy sensitivity of uveal melanoma in vitro,deliver LRP(lung resistance associated protein)antisense oligodeoxynucleotide(AS ODN)to reverse its drug resistance Methods:Sensitivity experiment were performed with 3,4,5 Dimethyliazol 2,5 diphenyl tetrazolium bromide(MTT)for the drugs 5 Fluroouraul(5 FU),Thiophosphramide(TSPA),Cispcatin(DDP),Adriamycin(ADM),Vinblastine(VLB),and Dacarbazine(DTIC)on the primary cultured uveal melanoma cells.
目的 :探讨体外培养的葡萄膜黑色素瘤细胞对不同化疗药物敏感性 ,并通过硫化嵌合型反义寡核苷酸 (AS -ODN)特异阻断肺耐药基因 (LRP)的表达逆转肿瘤多药耐药性。 方法 :通过原代培养葡萄膜黑色素瘤细胞 ,采用噻唑蓝 (MTT)法测定肿瘤细胞对氟脲嘧啶 ,噻替哌 ,顺铂 ,阿霉素 ,长春新碱 ,氮烯咪氨 6种药物体外敏感性。
10.Results:LSPC can inhibit the proliferation of B16 melanoma. The dosage of 120mg/kg·bw shows the most significantly difference in comparison with the control(p＜0.01). The inhibition rate of B16 melanoma reaches 55.3%,and the cell apoptosis analysis presents a sub- peak.
结果:一定剂量的LSPC能抑制黑色素瘤B16细胞生长增殖,且以120mg/kg·bw LSPC组最显著(p＜0．01),抑瘤率达55．3%,并有亚二倍凋亡峰。
