1.In this research, The pea MAR was used to construct T-DNA constructions, in which the pea MAR flanked the sides of gene gus and hpt (-MAR-gus-MAR-hpt、 -gus-MAR-hpt-MAR-) respectively, the pea MAR had no influence on gene expression in transient expression;
通过农杆菌介导转化水稻，GUS活性定量测定表明，MAR序列可以提高报告基因的表达水平，相对于MAR序列位于hpt基因表达框两侧，当MAR位于gus基因表达框两侧时GUS活性提高了两倍，并且能在后代中稳定表达，而不含MAR序列的对照转基因植株在T1代则出现了基因沉默；
2.A multi-gene plant expression vector pYLTAC747H-MAR-BADH-CMO-MAR was obtained by a new method based on Cre/LoxP recombination system and transferred into Agrobacterium Tumefaciens for plant transformation. The choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH) gene derived from Suaeda liaotungensis as well as matrix attachment region (MAR) isolated from tobacco.
本研究通过使用一套基于Cre／loxP重组系统的植物多基因表达载体构建系统，将辽宁碱蓬的CMO基因、BADH基因，以及烟草的核基质结合区(matrix attachment regions, MAR)序列，构建到同一表达载体上，得到可直接用于农杆菌转化的植物表达载体pYLTAC747H-MAR-BADH-CMO-MAR，为后续的杨树转化工作奠定了基础。
3.To further inquire into the functions of Mar-HSP70, we constructed Mar-HSP70-pET-28a (+) prokaryotic expression system, and successfully expressed the recombinant Mar-HSP70 in E.
而对于Mar-HSP70 mRNA水平仅对35℃热刺激才大量增加,并且这种上调主要集中在中枢神经、肝胰腺和肌肉组织中;
4.LVEDD was highly correlated to MND(r=-0.793, P
L VEDD分别与心肌细胞核密度及凋亡率高度相关 ( MND,r=- 0 .793 ,P
5.Cloning of the human β-globin MAR and construction of the pCAT-MAR expression vector
人珠蛋白MAR序列的克隆与表达载体pCAT-MAR的构建
6.The investigation suggests that Mar phenotype of Escherichia coli be determined by the levels of active efflux system acrAB gene expression, and the expression of acrAB gene should be regulated by mar operon.
本研究表明大肠杆菌acrAB表达水平决定了大肠杆菌多重耐药表型，mar可能调控acrAB的表达。
7.(2)Alkaline and peralkaline magmatism, mafic to felsic and associated carbonatites, at the Brazilian Serra do Mar, around the Parana Basin border, as well as in isolated spots in the border of Amazon Shield, in Bolivia.
在大陆的北部,出现局部的二叠—三叠纪拉斑玄武质岩墙。 (2)碱性和过碱性镁铁质至长英质岩浆活动和共生的碳酸盐岩,它们出现于巴西Serra do Mar、巴拉那盆地周边以及在亚马孙地盾周边和玻利维亚,呈孤立的小面积出现,时代上大部分属后阿尔必阶。
8.MAR,GMP-140 were decreased and EPM was increased both in treated group and control group after treatment, the decrease of GMP-140 in treated group was more significant than in control group.
治疗组和对照组治疗后,MAR降低和EPM水平升高; 治疗组GMP-140下降明显.
9.Our studies revealed that TM1-3 and AM4-3 not only李宏:植物 MARs 的 ARS 功能鉴定以及对 gus 基因表达活性的研究had higher ARS activity, but also displayed higher transformation frequency,plasmid stability and growth rate compared to their intact MARs, TM1 andAM4. These data present an important clue for further elucidating therelationship between MAR and ARS.
为进一步证明具有 ARS 功能的 MAR 增强外源基因表达作用,我们将具有 ARS 功能的 TM1 和 AM4 以及具有 ARS 功能的亚克隆片段 TM1-3和 AM4-3 分别插入植物表达载体 pBI121 中 gus 基因表达盒的两侧,通过转基因植株来检测这四个片段对 gus 基因表达的影响。
10.Results AOAP could increase the bone density and the levels of serum T/E2 and CT/PTH, raise the levels of TBV, TBS, TFS, LBGR, AFS, MAR and BFR, and reduce the level of TRS.
结果 AOAP能提高T/E2 、CT/PTH和骨密度 ,升高TBV、TBS、TFS、LBGR、AFS、MAR、BFR ,降低TRS。
