1.Specifically, we are interested in studying the regulation mechanisms at the molecular, cellular and organismal levels through studying several key components in mammalian and bacterial iron homeostasis: bacterial and mammalian iron acquisition, utilization, storage, and iron/oxidative stress adaptation/detoxification.
我们对几个重要的铁代谢的蛋白质进行系统研究，其研究重点是哺乳动物和微生物在分子、细胞和物种水平上铁代谢调节机制,即:哺乳动物和细菌的铁离子的获取、利用、存贮和铁离子/氧化物胁迫适应/解除分子及细胞调控机理.
2.Method: salmonella typhimurium mutagenicity test (Ames test), chromosome aberration (CA) test of cultivated cells of mammalian, micronucleus test in mice and mammalian bone marrow CA test were used.
方法：应用鼠伤寒沙门菌回复突变实验（Ames实验）、哺乳动物培养细胞染色体畸变（Chromosome Aberration, CA）实验、小鼠微核（Micronucleus, MN）实验和哺乳动物骨髓细胞CA实验。
3.The methylation and demethylationof cytosine in CpG dinucleotide plays an im-portant role in regulation of mammalian geneexpression. There are two kinds of promotersin mammalian genome:CpG-island promotersand CpG-deficient promoters. Two proteinfactors influence gene expression by interac-ting with methylated CpGs.
CpG双核苷酸中的胞嘧啶甲基化和去甲基化在哺乳动物的基因表达中有重要的调控作用．哺乳动物基因组中有两类启动子：CpG岛启动子和CpG缺乏启动子．两种蛋白质因子通过与甲基化CpG的相互作用影响基因表达，CpG岛在基因组分析中也有广泛的用途．
4.Second, some reports utilized the baculovirus as a gene delivery vehicle into mammalian cells and did not have any bad effect on the growth of mammalian cell.
因此，本论文中讨论一些影响蛋白质表现量与延长表现的参数如:培养基的更换、重复感染、病毒感染方式。
5.Adult mammalian neurons in peripheral nervous system (PNS) differ from that in central nervous system (CNS) in their response to injury, the former can successfully regenerate while the latter can not after axotomy.
成年哺乳动物外周神经系统(PNS)损伤后可以有效再生,而中枢神经系统(CNS)损伤后不能有效地再生连接。
6.Chronic Recording of the Compound Action Potential of the Mammalian Peripheral Nerves
哺乳类外周神经复合动作电位的慢性记录
7.Secondly, several modes of attenuation of Neo gene such as wild and mutant type of Neo gene, weak promoter lack of enhancer, frame-shift ATG, changed Kozak sequence and an intron in which Neo gene was placed were applied to construct expression vectors aiming at targeting mammalian integration sites that supported high levels of expression.
同时,本研究中以Neo基因为筛选基因,采用多种方式对其进行弱化,具体的弱化方式有:在Neo基因的起始密码子前添加不同阅读框架的ATG/改变其Kozak序列、将Neo基因置于内含子中以及使用活性降低的突变体。
8.An Easy Available Modified Method of Calcium Phosphate Co-precipitation With DNA for Transfection of Mammalian Cells
一种磷酸钙DNA共沉淀转化哺乳化物细胞较易成功的简易变通方法
9.Methods cDNAs encoding variable regions of heavy and light chains were isolated by RT-PCR from hybridoma cells secreting mouse monoclonal antibody (McAb) 4D11, and to be introduced into mammalian expression vectors pcDNA3. 1-AH and pcDNA3.1-Ak containing sequences of humanΥl andκconstant regions, respectively. The vectors were cotransfected into 293 FT cells.
方法通过RT-PCR方法从分泌抗乙型肝炎病毒preS1的鼠源单克隆抗体4D11的杂交瘤细胞中克隆出抗体基因的重链可变区(VH)、轻链可变区(Vκ)序列,并分别克隆到含有Υ1重链和κ轻链恒定区序列的pcDNA3.1-AH和pcDNA3.1-Ak质粒中,共转染人胚肾细胞变种(293 FT)细胞。
10.STUDIES ON RADIATION-DNA DAMAGE AND ITS REPAIR IN MAMMALIAN CELLS Ⅱ. HYDROXYAPATITE CENTRIFUGATION-FLUOROMETRIC METHOD FOR DETECTION OF DNA STRAND BREAK AND ITS REJOINING IN RAT BLOOD LYMPHOCYTES AFIER γ-RAYS IRRADIATION
哺乳动物细胞DNA辐射损伤与修复的研究Ⅱ 羟磷灰石离心-荧光法检测γ-线所致鼠血淋巴细胞DNA链断裂与重接
