1.To further demonstrate the interaction between GR and JAB1 and the effect of JAB1 on GR, PCR was performed to amplify GR LBD fragments and it was cloned into the bait vector pGBKT7 to create the plasmid pGBKT7 GR LBD. The plasmid was used as bait to screen a cDNA library constructed in the pACT2 vector. Transformants were plated on SD/-Ade/-His/-Leu/-Trp.
构建与Gal4 BD融合表达的载体pGBKT7 GRLBD ,与构建于pACT2载体上的人骨髓cDNA文库杂交 ,在SD/ Ade/ His/ Leu/ Trp选择培养板上培养 ,经X α gal检测 ,阳性克隆片段插入pGEM TVector载体 ,测序 ,再经酵母双杂交和GSTpulldown蛋白质结合实验验证其结合作用 ,应用反映GR转录活性的CAT报告基因检测JAB1对GR的调节活性 .
2.The studies suggest that JAB1 interacts with GR LBD expressed in COS7 cells in vitro and it potentiates the transactivation properties of GR.
JAB1加强GR转录激活的能力
3.One clone contained a cDNA insert with almost the entire coding sequence (amino acid 44-335) of human CSN5/JAB1, which is a coactivator of AP-1 transcription and the subunit 5 of CSN.
其中,JAB1又属于COP9复合物的第五组分,负责调节植物中的光信号、线虫中的幼虫发育、整合素信号、细胞周期调控和甾体类激素信号传导。 并且,其功能主要是加强AP-1(c-Jun/c-fos)与AP-1结合位点(TRE)即AP-1启动子区的结合力与稳定性,从而增强AP-1反式转录活性。
4.To jab or make a jab.
刺，戳，捅，猛击
5.“I think it is awake,” Martinez said now as he flipped a piece of sushi into his mouth. “Jab it with a chopstick to make sure.
“我想他是醒了，”马丁内兹一边拿快寿丝放自己嘴里一边说：“拿筷子捅一下试试。”
6.You have got a parry, in any case, so parry with one forearm and attack simultaneously with the knee and disengaged hand (chin jab or punch to the jaw).
在任何情况下你都需要格挡闪避，那么就用一侧的前臂偏转格挡，同时用膝盖或空着的另一只手进攻（掌跟打下颌或拳击）。
7.Recently, cytokine signal suppressor SOCS1(suppressor of cytokinesignaling-1) was found to be induced by LPS and CpG-DNA stimulation inmicrophages. SOCS1 also called SSI-1(STAT-induced STAT inhibitor-1)and JAB-1(JAK binding protein-1).
SOCS1 是细胞信号传导的一种负性调节因子,一些细胞因子如 IFN-γ、IL-4、 IL-6 等均可诱导 SOCS1 的产生,SOCS1 产生后随即可与 JAKs 结合,从而抑制 IFN-γ、IL-4、IL-6 等细胞因子刺激所产生的 JAK-STAT 信号级联反应的过度激活,进而抑制下游的信号传导。
8.The ball handler will often perform numerous flashy moves while attempting to drive to the basket, including crossovers, jab steps and other fake-out tricks.
持球者常常会在试图冲向球篮时施展无数花哨的动作，其中包括胯下运球、踮步和其它过人技巧。
9.Why are you always taking a jab at your friends?
你为何总是挖苦你的朋友？
10.But like the flu jab, R genes can be rendered useless if a pathogen mutates into a slightly modified form.
但是就像流感发作一样，如果病原变异为稍有不同的形式，R基因就会毫无用武之地。
