1.PARTⅠINDUCE A MODEL OF PASSIVE TRANSFERRED MYASTHENIA GRAVIS ON YOUNG MICEObjective: To induce an animal model of passive transferred myasthenia gravis (PTMG) on young C57BL/6 mice with different doses of nicotinic acetylcholine receptor monoclonal antibody-mAb35, to decide the effective dose of mAb35 required to induce PTMG, and to provide an animal model for further study of the pathogenesis and immunotherapy of human MG.
第一部分重症肌无力被动转移幼鼠模型的建立目的:利用乙酰胆碱受体(AChR)单克隆抗体-mAb35,以不同剂量腹腔注射给C57BL/6(B6)幼年小鼠,建立重症肌无力被动转移模型(PTMG),摸索成功建立动物模型的mAb35有效剂量,为进一步研究MG的发病机理和相应的免疫治疗提供实验动物模型。
2.KOAc can only induce calcium oxalate monohydrate (COM), while K3Cit and K2EDTA induce COM and calcium oxalate dihydrate (COD). The ability of multicarboxylates to induce COD formation was increased with increasing their concentrations. Calcium oxalate trihydrate (COT) was induced by K2Tart.
三元柠檬酸钾(K3Cit)和四元乙二胺四乙酸二钾(K2EDTA)可诱导二水草酸钙(COD)形成,且随着其浓度增加,对COD的诱导能力增加,而二元酒石酸钾(K2Tart)同时诱导了COM,COD和三水草酸钙(COT)生成.
3.The optimal medium for mature embryos to induce somatic embryos and cotyledons, and for hypocotyls of seedlings to induce adventitious buds was DCR+6-BA 1.0mg/L+TDZ0.003 mg/L+ NAA 0.1 mg/L.
DCR+6-BA 1.0mg／L+TDZ0.003 mg／L+NAA 0.1 mg／L是成熟合子胚诱导体胚、子叶和下胚轴诱导不定芽发生的最佳培养基；
4.Appending 15% (v/v) coconut water (CW) can enhance the induce rate and advance the growth of the PLB. In MS+BA 5.0+CW 15% medium adapt to induce PLB from leaves, inducing rate is 38%.
MS+BA 5.0+CW 15％培养基较适合诱导叶片产生原球茎，诱导率最高达到38％。
5.MS medium containing both 5mg/L BA+2mg/L NAA or 5mg/L BA+3mg/L NAA could induce much callus and adventitious buds from El explants, and the latter medium could induce much more callus and buds than the former, but with more degree of browning.
MS+5mg／L BA+2mg／L NAA和MS+5mg／L BA+3mg／L NAA均可诱导胚小叶外植体产生愈伤和大量不定芽，后者产生的愈伤和芽相对较多，但褐化稍重。
6.GroupⅠto inject intravenous esmolol 300μg·kg -1·min -1 with Baxter of USA AS40A micro-pump 4 min before induce, to inject esmolol 200μg·kg -1·min -1 in anesthesia induce, kept on till 10 min after intratracheal intubation.
组Ⅱ ,于诱导前 4min以美国百特微泵静注艾司洛尔 30 0 μg·kg-1·min-1,诱导时改用艾司洛尔 2 0 0 μg·kg-1·min-1,至插管后 10min停药。
7.Conclusion The expression of mice DSPP on BM-MSCs by gene transfection can induce theexpression of tooth development-associated gene Pax9 and enhance the formation of mineralized nodules,which demonstrated that DSPP gene maybecontribute to induce odontogenic differentiation of BM-MSCs.
结论BM-MSCs 转基因表达DSPP 能够增强其矿化能力,并诱导表达牙齿发育相关基因的表达,提示DSPP 可能在牙齿发育早期有一定作用。
8.The costimulatory signal mediated by 4-1BB molecule could mainly induce the proliferative response of CD8 + T cells, while that mediated by CD28 molecule could induce the activation of CD4 + T cells mainly.
4 1BB分子所介导的协同刺激信号 ,在CD8+ T细胞活化及增殖中发挥了更为重要的作用 ; 而CD2 8分子所介导的协同刺激信号则更有利于CD4 + T细胞的活化
9.(2) induce apoptosis of RMC through increasing the ratio of Bax to Bcl-2 gene expression. (3) induce ECM degradation through increasing the ratio of MMP2 to TIMP1 gene expression.
PC-1NF融合蛋白可通过上调促进凋亡基因Bax和下调抑制凋亡基因Bcl-2而诱导大鼠系膜细胞凋亡并上调促进细胞外基质降解的MMP2和抑制细胞外基质降解的TIMP1之间的比值，促进细胞外基质降解。
10.Although azimilide could induce EAD and R-on-T premature beats too, it could not induce Tdp.
azimilide不增加TDR和不形成跨壁折返,但可诱发EAD和R-on-T早搏。
