1.Tetracycline (10 -6 ～10 -4 mol·L -1 ) was used to incubate HUVECs for 1 h before stimulation with TNF α ( 1 μg·L -1 ) or LPS ( 5 mg·L -1 ) and co incubate HUVECs with TNF α or LPS for 4 h or 18 h.
用四环素 (10 -6～ 10 -4 mol·L-1)预孵育内皮细胞 1h再与TNF α(1μg·L-1)或LPS(5mg·L-1)共同孵育4h或 18h ;
2.We found that, add specimen to polystyrene bead with HBsAg incubate at 45℃ for 1～1.5 hrs, then add labeled HBsAg again incubate at 45℃ for 1.5～2 hrs would got the highest rate of combination.
我们认为在包被HBsAg的固相载体中加入待检血清样品,45℃孵育1～1.5小时,加入标记抗原,45℃孵育1.5～2小时,结合率最高。
3.analysed by means of SDS polyacrylamide gel electrophoresis (SDS PAGE) and followed by Western blotting. The membranes were incubate with biotinylated wheat germ agglutinin (WGA/Bio) and avidin biotin horseradish peroxidase complex (ABC kit) respectively, then developed with 4 chloro 1 naphthol.
血小板样品经 SDS-聚丙烯酰胺凝胶电泳 (SDS- PAGE)后 ,再转移至硝酸纤维素膜上(Western blotting) ,用生物素化的麦胚凝集素 (WGA/ Bio)偶联辣根过氧化酶复合物试剂盒 (ABC kit)分步孵育 ,再以 4-氯 - 1-萘酚显影。
4.We steadily exert our company spirit of Enterprising, Trustworthy, Professional and Developing, and incubate a company culture that cares and shares,establishing in the society an image of a company that is both innovating and contributing .
坚持发扬敬业、诚信、专业、发展的企业精神，培育人企合一、亲情关怀的企业文化；树立创新图强、报效祖国的社会形象。
5.2. p73 dyeing: generally, slice is missed out wax to hydrate, by antigen repairing, drop in non - antigen anminal serum, incubate 5 minutes in normal room temperature ,wash up in PBS liquid.
Zi…3染色：切片常规脱腊至水化后，经抗原修复，滴人非抗原性动物血清O0％正常羊血清）封闭，室温下孵育5分钟，PBS液冲洗一次。
6.MethodsTo digest the passage 2 and 7 DPCs in log growth phase and to adjust the cell concentration to 3×105 cells/mL and incubate the DPCs to 24 hole cell culture flask.
方法消化对数期生长的第2代和第7代DPC,调整细胞浓度为3×105细胞/mL,接种于24孔细胞培养板;
7.After dehydroepiandrosterone derivative and dehydroepiandrosterone sulfate were used to incubate for 24 h, the surviving rate of hippocampal neurons was increased, the activity of GSH-Px was increased, LDH activity and MNA content were decreased compared with A β 25-35 group.
海马神经元与不同浓度脱氢表雄酮硫酸酯和脱氢表雄酮唾液酸苷共孵 2 4h后 ,能改变Aβ2 5-3 5引起的损伤 ,使细胞存活率显著升高、GSH Px活性升高、LDH活力和MDA含量降低。
8.large-footed short-winged birds of Australasia; build mounds of decaying vegetation to incubate eggs.
澳大利亚大脚短翅的鸟；用腐草建造土堆以在其中孵蛋。
9.Take a portion of the same colonies as under 3.8.1 by means of a wire and stab inoculate a glucose agar tube. Incubate at 37 ?1 °C for 24 h.
用接种针挑取与3.8.1实验相同的菌落的一部分接种至葡萄糖琼脂试管.37?1°C培养24小时。
10.6. Detection of CD4 absolute counts: Pipette 20l of TriTEST CD4/CD8/ CD3 reagent and add 50l anticoagulated whole blood into the bottom of the TruCOUNT Tube using reverse pipetting and incubate for 15 minutes in dark at room temperature (20 - 25 ).
6．CD4绝对值计数：将20μl CD4／CD8／CD3三色单抗加入绝对计数管中，加入50μl抗凝全血，室温避光15min，加入免洗溶血素450μl，室温避光15min，FACS Calibur流式细胞仪检测，MULTISET软件自动分析CD4~+、CD8~+、CD3~+淋巴细胞绝对值及CD4／CD8比值。
