1.MethodsComparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) were performed to detect the known small marker chromosome in this patient.
方法:应用比较基因组杂交(comparative genomic hybridization,CGH)和荧光原位杂交(fluorescence in situ hybridization,FISH)技术对1例小标记染色体患者进行分子细胞遗传学检测。
2.There are many different techique to detect the difference of gene expression between the cells with different biological phenotypes, such as Expressed sequencing tag(EST), Serial analysis of gene expression(SAGE), subtractive hybridization, mRNA differential display (DD-RTPCR), cDNA representative difference analysis (RDA), suppressive subtractive hybridization(SSH), and cDNA microarry.
当前研究不同生物学表型细胞间基因表达差异的主要技术手段有表达序列标签(EST)、基因表达系列分析(SAGE)、差异展示(DD-RTPCR)、代表性差异分析(RDA)、抑制性消减杂交(suppressive subtractive hybridization，SSH)、以及基因芯片基础上的技术(chip-based approach)等。
3.METHODS: Chromosomal gains and losses in 39 specimens of LSCC were identified by comparative genomic hybridization (CGH), the association between chromosomal imbalances in LSCC and smoking was analyzed.
方法:采用比较基因组杂交(comparative genomic hybridization,CGH)技术对39例原发性肺SCC的染色体扩增和缺失进行检测,并分析吸烟与肺SCC染色体不平衡之间的关系。
4.Comparative genomic hybridization (CGH) was employed to detect the genomic imbalance (DNA loss or amplification) in 16 RMS cases.
应用比较基因组杂交(comparative genomic hybridization,CGH)技术检测16例RMS的不平衡即DNA的丢失或扩增。
5.Objective To set up a fluorescent in situ hybridization(FISH) based method to detect the gene-deleted female carriers of Duchenne/Becker muscular dystrophy (DMD/BMD).
目的 建立应用荧光原位杂交 (fluorescent in situ hybridization,FISH)方法检查进行性假肥大性肌营养不良 (Duchenne/ Becker muscular dystrophy,DMD/ BMD)患者家系中女性亲属是否为携带者的方法。
6.A METHOD OF IN SITU HYBRIDIZATION FOR DEMONSTRATION OF THE TSH β GENE EXPRESSION IN RAT PITUITARY
A METHOD OF IN SITU HYBRIDIZATION FOR DEMONSTRATION OF THE TSH β GENE EXPRESSION IN RAT PITUITARY
7.METHODS: Chromosomal imbalances were studied in 30 GCA resection specimens and 7 metastatic LN lesions by comparative genomic hybridization (CGH).
方法:应用比较基因组杂交技术(comparative genomic hybridization,CGH)来分析原发性贲门癌患者30例及其相应淋巴结转移灶7例中染色体基因组的变化.
8.In order to analyze the molecular mechanism for salt tolerance in P Euphratica, salt-related genes were screened through Suppressed Subtractive Hybridization (SSH) with the results as follows:
为了研究胡杨耐盐的分子机制，通过抑制性扣除杂交（Suppressed subtractive hybridization, SSH）技术从胡杨中筛选与耐盐性相关的基因。 结果如下：
9.17 strains were positive hybridization with probe Slb,2 strains were positive hybridization with probe Elb,1 strains were positive hybridization with probe Elc,5 strains were positive hybridization with probe Eld,1 strains were positive hybridization with probe Ele,11 strains were positive hybridization with probe El;
31株耐EMB菌株中,13株Elb杂交阳性,1株Elc杂交阳性,5株Eld杂交阳性,1株Ele杂交阳性,11株与El探针杂交。
10.To utilize rapeseed male-sterile lines,increase the affinity of cruciferae hybridization and breed new vegetable species,the studies conducted the affinity experiments of hybridization, investigated the cytoplasm effection of hybridization,growth vigor,yield and main nourisgment elementsts of tender flower stelk.
本研究的主要目的是探索利用油菜雄性不育系，提高十字花科种间杂交亲和性，以培育新的蔬菜品种。 进行了种间杂交亲和性试验，调查了种间杂交的亲和性，细胞质效应和杂交组合的生长势。
