1.It contains 43 restriction sites for Aat I,Aat II,Bgl I,Dpn I,Dpn II,EcoR II,Hae I,Hae II,Hae III,Hinf I,Hpa II,Mae II,Mae III,Nba I,Oxa I and Sst IV. The sequence homology for the chitinase(Class I)mature protein genes between V. sesquipedalis and V. unguiculata,Phaseolus vulgaris,Pisum sativum,Nicotiana tabacum,Triticum aestivum and Oryza sativa is decreased respectively.
具 Aat I、Aat II、Bgl I、Dpn I、Dpn II、Eco R II、Hae I、Hae II、Hae III、Hinf I、Hpa II、Mae II、Mae III、Nba I、Oxa I和 Sst IV酶切位点 43个 ;
2.PCR products of 1720bp were digested with restriction endonuclease Hae Ⅲ and the results showed that M41,Connecticut,D41B shared the same RFLP pattern,whereas Arkansas or D41A had the different RFLP pattern.
用引物B时 ,所有 8个IBV毒株均得到与预计大小相符的目的产物 (2 2 8bp)。 对 172 0bp的PCR产物用限制性内切酶HaeⅢ进行酶切 ,结果得出 3个不同的RFLP图谱 ,其中M41、Connecticut、D41B具有相同的HaeⅢ酶切图谱。
3.The results showed that 2075 bp PCR products digested with HaeⅢ demonstrated polymorphisms in four populations which were at Hardy-Weinberg equilibrium (P>0.05). Moreover, the frequencies of allele B/A of QQ, AQ, DQ and LQ populations were 0.232/0.768、0.333/0.667、0.178/0.822 and 0.181/0.819.
结果表明,QQ及AQ、DQ、LQ群体H-FABP-HaeⅢ基因座的207 5 bp的PER产物被Hae Ⅲ限制性酶消化后表现多态性,它们的等位基因AIB频率分别为:0．232／0．768、0．333／0．667、 0．178／0．822和0．181／0．819,且均处于Hardy-Weinberg平衡状态。
4.PCR products of 1720bp digested with restriction endonuclease Hae Ⅲ showed that M41,Connecticut ,D41B shared the same RFLP pattern, whereas Arkansas or D41A had different RFLP pattern.
对1720bp的PCR产物用限制性内切酶Hae Ⅲ进行酶切,结果得出三个不同的RFLP图谱,其中M41、Connecticut、D41B具有相同的HaeⅢ酶切图谱,Arkansas和D41A则分别具有互不相同的图谱;
5.Objective: The present study aims to get some basic information about exon8 mutations of C1 esterase inhibitor (C1INH) gene deficiency which result in hereditary angioedema(HAE), and to expect to find new mutation by analyzing 33 Asian HAE patients.
目的：通过基因测序分析33例亚洲遗传性血管性水肿(HAE)患者C1酯酶抑制剂(C1INH)基因第八外显子(Exon8)的变异情况，了解亚洲人群C1INH变异基因的特点并期望发现新的基因变异。
6.RESULTS: The mean survival period of all 5 cases were 14 7 months,among them group HAI being 9 7 months,group HAE 16 3 months,while one year survival rates being 23 1% and 62 8% for the former and later respectively,(P
结果：56例平均生存期为14?7月，其中HAI为9?7月，HAE为16?3月，一年生存率HAE组明显高于HAI组，分别为62?8％和23?1％（P＜0?05）；
7.The association between β HaeⅢ polymorphisms located in the promoter region of β fibrinogen gene, plasma fibrinogen levels and coronary heart disease
β纤维蛋白原基因启动子区HaeⅢ多态性和血浆纤维蛋白原浓度与冠心病的相关性研究
8.Clinial comparison of treatment for acute mononuclear leukemia with HAE,DAE and MAE protocols
HAE、DAE、MAE方案治疗急性单核细胞白血病的临床比较
9.Twelve strains representing 10 species were evaluated by using PCR-RFLP analysis. ITS was amplified and digested by four base restriction enzymes(AluⅠ,HaeⅢ,HpaⅡ,TaqⅠ). The results showed that the length of ITS varied among isolates ranging from 658 to 705?
以ITS1和ITS4为引物对该属10种 12个菌株的核糖体DNA转录间区 (ITS)进行了PCR扩增 ,4种内切酶 (AluⅠ ,HaeⅢ ,HpaⅡ ,TaqⅠ )酶切 .
10.Methods Abstracted HCV RNA from 69 HCV infectious serum specimens and HCV 5′non conding region(5′NCR) was amplified by reverse transcription nested PCR. The PCR products were digested by 4 restriction enzymes(BstUⅠ?NciⅠ?HaeⅢ and RsaⅠ) for restriction fragment length polymorphism(RFLP) analysis.
方法 提取 69份血清标本的HCVRNA ,通过逆转录套式PCR(RTnested PCR)扩增 5′端非编码区 (5′NCR) ,以 4种限制性内切酶BstUⅠ、NciⅠ、HaeⅢ、RsaⅠ对扩增产物进行限制性片段长度多态性分析 (RFLP) ,依据不同的酶切电泳图谱确定HCV基因型。
