1.Diagnosis of CPV infection should be based on the study of P32 protein. Pairs of primers were designed with Primer 5.0 according to Goat pox virus (GTPV) P32 sequence published in Genbank.
本实验根据GenBank中发表的山羊痘病毒(Goat pox virus,GTPV)P32基因序列,利用软件Primer 5.0分别设计了P32鉴定和表达用引物。
2.The FIC index of tetracycline with ofloxacin or tylosin on Y-goat was 0.375.
红霉素与泰乐菌素对Y goat的联合药敏指数为1.5,是无关作用; 四环素与氧氟沙星、泰乐菌素对Y 98的联合药敏试验指数均为0.375,是协同作用。
3.Goat and sheep pox diseases are serious infectious diseases caused by infection of capripoxvirus (CPV).
山羊痘和绵羊痘病(Goat and sheep pox disease)是由羊痘病毒(Capripoxvirus, CPV)感染山羊和绵羊等偶蹄动物引起的一种烈性传染病,病畜以发热、全身性起痘为典型特征。
4.PCR-RFLP was applied to analyze the polymorphism of CSN1S2 gene in 170 goats that comprised of five goat breeds, namely Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat. A 310 bp -long PCR product was digested with Alw26Ⅰ and demonstrated polymorphism in five goat populations that were all at Hardy-Weinberg equilibrium (P>0.05).
利用PCR RFLP技术对西农萨能奶山羊、关中奶山羊、陕南白山羊、安哥拉山羊和波尔山羊5个品种的170个个体的αs2酪蛋白(CSN1S2)基因进行多态性分析,结果表明:扩增大小为310bp的片段经限制性内切酶Alw26Ⅰ酶切后表现多态,且5个山羊品种该基因座位均处于Hardy Weinberg平衡状态。
5.And PCR-RFLP and PCR-SSCP was used to detect genetic diversity of five goat breeds (including Xinong Saanen dairy goat, Guanzhong dairy goat, Shaanan White goat, Angora goat and Boer goat) .
同时，还利用PCR-RFLP和PCR-SSCP方法分别检测了西农萨能奶山羊、关中奶山羊、陕南白山羊、安哥拉山羊和布尔山羊5个山羊品种共计175个个体的10个基因座的遗传多态性，为山羊品种资源保护和畜牧业的发展提供资料。 本论文通过上述研究首次取得了以下结果：西农萨能奶山羊的PCR-RFLP研究
6.At shed raising conditions, the estrous behavior of Boer goat compare to Guanzhong dairy goat were determined in this study. The results showed that the estrous behavior of Boer goat was no distinctness compare to Guanzhong dairy goat. The duration of Estrous of Boer goat and Guanzhong dairy goat were 26.00±8.98hr and 40.93±8.39hr respectively (P<0.0.1).
本文通过对两个引进品种——波尔山羊与关中奶山羊发情行为比较观察,结果发现,波尔山羊发情期三个阶段,发情初期、发情末期,尤其在发情盛期的行为没有关中奶山羊的明显,发情持续时间为26.00±8.98hr,短于关中奶山羊的40.93±8.39hr(P<0.0.1)。
7.The result of x ~2 fitness test of primer 2 indicated that Jining grey goat, Wendeng dairy goat, Liaoning cashmere goat, Beijing native goat and Boer goat all kept in Hardy-Weinberg equilibrium (P>0.05).
对济宁青山羊、文登奶山羊、辽宁绒山羊、北京本地羊、波尔山羊5个山羊品种具有多态性的引物的扩增片段进行x~2适合性检验:引物2扩增片段在这5个山羊品种群体中都保持了Hardy-Weinberg平衡(P>0.05);
8.The production situation of angora goat and mohair in the world and the effect and research progress of improving goat breeds in different anreas by hybridization with angora goats since 1985 in China ate brieflyexpounded. It shown that angora goat X Zhongwei F2 has attained the traitpossessed by F3 of the other society. Zhongwei is a good variety for breedingChinese mohair goat breeds.
简要阐述了世界安哥拉山羊与马海毛生产概况，及1985年以来中国利用安哥拉山羊杂交改良不同地方山羊品种的效果和研究进展，表明：安×中（Angoragoat×Zhongweigoat）F2代已达到其它组合F3所具备的品质特征，中卫山羊是培育中国马海毛山羊品种的优良种。
9.The item quests for the experiment of the native goat cross to Boer Goat in order to solve the problem of being short of fine varieties and to develop the goat industry.
为了解决榆林地区肉山羊发展缺乏良种的问题,探索榆林肉羊发展的模式,以便指导本地区养羊业的发展,通过引进布尔山羊,用布尔山羊的种公羊作为杂交父本,与本地普通山羊母杂交羊开展了布尔山羊与榆林地方普通山羊杂交效果的研究,试验结果表明:(1)布尔山羊在本地区具有较好的适应性。
10.Comparative analysis showed that the homologies of DRB3 gene among Human, Bovine, Mongolian sheep, Mongolian goat, Kazakh goat, Spanish goat, Small-tailed Han sheep, Large-tailed Han sheep,. Shandi sheep, Wadi sheep, Dorsat sheep and Texel sheep are very high.
3.人类、牛、蒙古山羊、蒙古绵羊、哈萨克山羊、西班牙山羊、小尾寒羊、大尾寒羊、山地绵羊、洼地绵羊、道赛特羊和特克赛尔羊的DRB3基因核苷酸序列以及氨基酸序列之间的同源性比较高。
