1.The main chain is made up of Gal and Glc, the inner is (1--3) -linked-Gal, the border is (1--6)-linked-Gal , (1--6)-linked-Gal and (1--3)-linked-Glc residues are substituted at 6-0. On average, there is 4 branch among 10 main chain residues.
主体结构由Gal与Glc组成，其中(1→3)Gal构成主链的核心结构，(1→6)Gal构成主侧链的边缘，(1→6)Gal在6-0处有分枝，平均每10个己糖残基有4个分枝；
2.Lectin BS-I-B4 is used to mimic the human xenogeneic natural antibody since the capacity of binding Gal-α1,3-Gal. So it can be used to select Gal-α1,3-Gal mimikers.
西非单叶豆凝集素(BS-I-B4)能结合α-1,3半乳糖(Gal-α1,3-Gal),因而可以用此凝集素模拟人天然抗体来进行α-1,3半乳糖(Gal-α1,3-Gal)模拟物的筛选。
3.Sequence analysis of Gal-4 eDNA showed that the cDNA of Gal-4 gene con- sists of 321 bp,encoding Gal 4 polypeptide of 63 amino acids,which comprised of signal peptide with 20 amino acids at N’terminus,and mature peptide with 38 amino acids.
序列分析结果表明,获得的广西黄鸡Gal-4基因大小为321 bp,推导的Gal-4由63个氨基酸组成,其中N端20个氨基酸为信号肽,C端38个氨基酸组成Gal-4的成熟肽。
4.Lectin dot-blotting with horseradish peroxidase-labeled WGA was performed for the bile samples from each of the 35 cases of BDC and BBD. ResultsThe mRNA levels of ST3Gal-Ⅲ and ST6Gal-Ⅰ elevated in BDC(P
结果胆管癌组织中ST3Gal Ⅲ和ST6Gal ⅠmRNA表达均增强 (P
5.It is found that X-gal has a powerful ability to quench the fluorescence of AAL. The binding constant of X-gal is 7.33×10~(3)L·mol~(-1),the number of binding sites is 8.3,and the binding distance is 2.79 nm which are obtained based on the theory of Foster spectroscopy energy transfer.
X-gal与AAL的结合强烈猝灭AAL的荧光,且X-gal的紫外吸收光谱与AAL的荧光发射光谱有一定程度的重叠,由此得出了其反应的结合常数(Kb=7.33×103L.mol-1),结合位点数(n=8.3)及作用距离(r=2.79 nm)。
6.Results ST3Gal-Ⅲ,ST6Gal-Ⅰand GnT-Ⅴexpression was all enhanced in BDC(P
结果 胆管癌组织中ST 3Gal Ⅲ、ST6Gal Ⅰ和GnT Ⅴ表达均增强 (P
7.The key feature of the GAL4 system is that the GAL4 gene and UAS-target gene are initially separated into two distinct transgenic lines.
GAL4/UAS系统的关键点在于:GAL4基因和UAS-靶基因分别存在于两个转基因系中。
8.Objective To study the combinatorial effects of small interfering RNA (siRNA) and antisense oligonucleotides (ASOs) targeting alpha 2,6 sialyltransferase (ST6Gal I) on the cell adhesion and invasiveness of human cervical carcinoma cell line, HeLa with over-expressing ST6Gal Ⅰ.
目的探讨人α2,6-唾液酸转移酶(ST6GalⅠ)小分子干扰RNA(siRNA)及其与反义寡核苷酸(ASO)联合应用对ST6GalⅠ高表达的宫颈癌HeLa细胞的粘附和侵袭力的影响。
9.analysis indicated that HP I was composed of Fuc, Glc, and Gal in the molar ratio of 0.423: 1: 2.110, and HP II was composed of Glc and Gal in the molar ratio of 11.529: 1.In the structure analysis of HP and HP , partial hydrolysis with acid, periodate oxidation, Smith degradation, I.
分析表明，单糖组成分别为Fuc、Glc、Gal与Glc、Gal，摩尔比分别为0.423：1：2.110与11.529：1。 HPⅠ与HPⅡ的结构分析采用了部分酸水解，高碘酸氧化，Smith降解，I.
10.Artocarpus hypargyreus lectin(AHL) were purified by affinity chromatography on human serum γ golubin sepharose 4B and on Gal sepharose 6B respectively The results showed that there were no significant differences as regards the purity,hemagglutinating activity and recovery rate of AHLs purified by these two methods But Gal sepharose 6B affinity chromatography is safer simpler,quicker and cheaper than human serum γ globulin sepharose 4B Thus Gal sepharose 6B affinity chromatagraphy is a good method for the purification of AHL
分别采用人血清γ球蛋白－Sepharose4B亲和层析及半乳糖（Gal）－Sepharose6B亲和层析来分离纯化白桂木凝集素（AHL）。 结果：两种方法提取的AHL在纯度、凝集活力及回收率上均无明显差别。
