1.Meanwhile, non-regulated enzymes witch catalyzing reversiblereactions such as fructose 1,6-bisphosphate aldolases (FBA) which catalyzing the cleavage offructose 1, 6-bisphosphate (FBP) exerted significant control over carbon flux.
而传统上不被重视的催化可逆反应的果糖-1,6-二磷酸醛缩酶(Fructose-1,6-bisphosphate aldolase, FBA,EC4.1.2.13)活性降低后,明显抑制了转基因植物的光合碳流量,暗示非调控酶在加速碳固定反应方面有着非常大的潜力。
2.As a preliminary stage of this study, three genes coding for three important Calvin cycle enzymes, i.e. triosephosphate isomerase (TPI), fructose 1,6 bisphosphate aldolase (FBP aldolase), and fructose 1, 6 bisphosphatase (FBPase), respectively, have been cloned into one plasmid, pTrcFAT, which is controlled by promoter trc . Successful co transcriptional expression of these three genes resulted in high yields of these enzymes under the induction of 0.25 mmol/L IPTG.
作为研究的初始阶段 ,将编码丙糖磷酸异构酶、果糖 1,6 二磷酸醛缩酶及果糖 1,6 二磷酸酶的 3个基因构建进一个由启动子trc控制的表达质粒 pTrcFAT ,成功地在大肠杆菌中实现了上述 3个基因的活性共表达。
3.The rates of oxidation of monosaccharides equal correspondingly to their rates of enolization. The rate of enolization of fructose is 11. 3 times that of glucose. This might lead to that fructose reacts much more easily than glucose with Tollens, Fehling or Benedict reagent.
通过对果糖和葡萄糖的氧化反应机理的探讨,烯二醇的形成是单糖氧化的决定速率步骤,单糖的氧化速率等于它的烯醇化速率,果糖的烯醇化速率是葡萄糖的11.3倍,解释果糖在与Tol-lens,Fehling,Benedict等氧化剂的反应中比葡萄糖更为活泼
4.Fructose decomposition consisted of two successive reactions, first from fructose to 5-HMF, then from 5-HMF to levulinic acid.
果糖分解反应主要由果糖分解为中间产物5-HMF和5-HMF进一步分解成LA两步组成,乙酸的加入加快了第一步反应速度,而对第二步反应无明显影响,因而中间产物5-HMF收率可高达83%;
5.The diluentcontaining glucose and Fructose was superior to that containing anyone of them ,and the diluent containing gentamicin was superior to thatcontaining penicillin and streptomycin for preservation Boer goatspermatozas. No LII was the ideal diluent formula consisting ofGlucose ,Fructose, Sodium citric acid ,EDTA, Gentamicin.
分析结果表明:以葡萄糖:果糖=1:1 混合物作为稀释液营养剂的保存效果较以其中任何一种作为营养剂的保存效果好:以庆大霉素作为抗菌素的保存效果比以青、链酶素作为抗菌素的效果好,因此筛选出了在常温条件下保存布尔山羊精液有理想效果的稀释液配方*,其主要成份为:葡萄糖、果糖、柠檬酸钠、EDTA、庆大霉素。
6.Fructose+L-Arginine fed (FFR+L-Arg) given with high fructose-diet and 2.25% L-arginine solution, L-Arginine- fed(L-Arg) given with irregular diet and 2.25% L-arginine solution.
果糖组(FFR)喂以60%的高果糖饲料,对照组(Control)喂以热量相同的普通饲料,果糖+L-精氨酸(FFR+L-Arg)组给予高果糖饲料喂养的同时饮水中加入L—精氨酸(浓度2.25%),L-精氨酸(L-Arg)组给予普通饲料喂养的同时饮水中加入L-精氨酸。
7.The substrate fructose-6-phosphate(F6P),the products fructose-1,6-bisphosphate(FBP) and phosphate(Pi) protected PFP against the DEPC modification,suggesting that the modified histidine residue resides at active sites.
底物F6P、产物果糖1,6-二磷酸(FBP)、P i及激活剂果糖2,6-二磷酸(F2,6BP)均可保护酶免被DEPC失活,底物PP i却没有保护作用.
8.The content of sucrose, glucose, fructose, starch and the activities of sucrose-metabolizing enzymes, including acid invertase(AI), neutral invertase(NI), sucrose synthase (SS) and sucrose phosphate synthase(SPS), were studied in sarcocarp of Elizabeth and Wuyin No. 1 muskmelon varieties. Physiological mechanism of sugar accumulation in muskmelon fruit was systemically analyzed.
以伊丽莎白和乌引1号2个厚皮甜瓜品种为试材,测定了不同发育时期果实中的糖、淀粉含量以及蔗糖代谢相关酶—酸性转化酶(AI)、中性转化酶(NI)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)的活性变化等,并对甜瓜果实糖积累的生理机制进行了系统分析。
9.This recommendation was soon reinforced by the food industry, which began selling cookies, chips and other products that were low in fat but often high in sweeteners such as high-fructose corn syrup.
这项建议迅速由食品工业界所强化,他们开始贩售饼乾、马铃薯片以及其他脂肪含量低、但却往往含有大量甜味剂(如高果糖玉米糖浆)的产品。
10.Results: [1] Gcp was composed of fructose, xylose, and glucose (24∶1∶4),with its average molecular weights about 7.5kD.
结果Gcp分子量约为7.5kD,单糖组成与比例为:果糖:木糖:葡萄糖=24:1:4。