1.After analyzing the 16S rDNA plus ITS (Inter Transcript Space) between 16S and 23S rDNA region of these isolates with PCR-RFLP (Restriction Fragment Length Polymorphism) method, we’ve got altogether 19 genotypes in these isolates. 16 of them belong to PCA producing isolates.
利用限制性片段长度多态性的分析(Restriction Fragment Length Polymorphism, PCR-RFLP)方法,根据该菌群核糖体16S rDNA以及16S rDNA与23S rDNA之间的间隔区域(Inter Transcript Space, ITS)的HaeIII酶切片段类型,可将所获得的假单胞菌群分为19种不同的基因型,其中合成PCA的菌群可分为16个不同的基因型。
2.LABELING McAb 3H11 AND ITS Fab FRAGMENT WITH ￣(211)At AND THEIR IMMUNOREACTIVITIES AND INJURY EFFECTS ON HUMAN GASTRIC CANCER CELLS
LABELING McAb 3H11 AND ITS Fab FRAGMENT WITH ~（211）At AND THEIR IMMUNOREACTIVITIES AND INJURY EFFECTS ON HUMAN GASTRIC CANCER CELLS
3.Objective To express human single-chain variable fragment (ScFv) antibody against non-structural protein 3 (NS_3) of hepatitis C virus in E. colt.
目的在大肠杆菌XL_1－Blue中表达可溶性的抗HCV非结构蛋白NS_3的人源单链可变区抗体（single－chain variable fragment antibody，SCFV）。
4.MethodsFour hunderd and three patients with CHD and 500 healthy controls without CHD were investigated. HPA-2 polymorphisms in GPIbα gene were checked by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).
方法选择403例确诊冠心病的患者和500例无心脏病史的健康体检者作研究对象,运用多聚酶链反应—限制性内切酶片段长度多态性技术(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)进行HPA-2多态性检测.
5.TUMOR UPTAKE AND THERAPEUTIC EFFECT OF ASTATINATED INTACT McAb 3H11 AND ITS Fab FRAGMENT FOR HUMAN GASTRIC CANCER XENOGRAFT IN NUDE MICE
TUMOR UPTAKE AND THERAPEUTIC EFFECT OF ASTATINATED INTACT McAb 3H11 AND ITS Fab FRAGMENT FOR HUMAN GASTRIC CANCER XENOGRAFT IN NUDE MICE
6.Univalent antibody fragments, such as single chain variable fragment (ScFv), do not cross-link AChR molecules or bind complement, and therefore do not cause AChR loss;
由抗AChR MIR抗体制备的单链抗体（single chain variable fragment, ScFv）做为单价片段既不与AChR交叉结合，也不引起补体经典途径的激活，因此不会引起AChR的丢失。
7.Following the principle of case control,polymerase chain reaction and restriction fragment length polymorphism were used to observe polymorphisms of ACE2 and genotyping was confirmed by gene sequencing in 20 subjects selected from the EH patients randomly.
按照性别分为两组,采用病例对照的原则,应用聚合酶链反应和限制性内切酶片段长度多态性(polymerase chain reaction and restriction fragment length polymorphism,PCR-RFLP)的方法检测ACE2基因G9570A多态性,并随机抽取20份标本进行基因测序以核实基因分型。
8.A NEW APPROACH TO GENE DIAGNOSIS OF DUCHENNE/BECKER MUSCULAR DYSTROPHY──AMPLIFIED FRAGMENT LENGTH POLYMORPHISM
A NEW APPROACH TO GENE DIAGNOSIS OF DUCHENNE／BECKER MUSCULAR DYSTROPHY──AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS
9.Methods The blood samples were chosen from 148 Sherpas in Tibet high altitude and 90 Han nationality healthy people in Guangdong province, and from which genomic DNA was extracted. The single nucleotide polymorphisms of 1772(C→T) and 1790(G→A) in exon 12 of HIF1A gene were examined by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR).
方法选取世居西藏高原夏尔巴族148人及广东汉族健康个体90人的血样,提取白细胞基因组DNA,聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)检测HIF1A基因第12外显子1772(C→T)、1790(G→A)的单核苷酸多态性,分析其基因多态性特征。
10.This article reports the data of restriction fragment length polymorphism (RFLP)on human Duchnne muscular dystrophy (DMD) site in a Chinese DMD family. DNAsfrom the family members were digested with a variety of restriction endonucleases andprobed with 9 kinds of probes obtained from Xp21 region of X chromosome. 13 polymorphic restriction sites on Xp21 were thus detected.
应用限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)分析法对一个Duchenne型肌营养不良症(Duchenne muscular dystrophy. DMD)家系进行了遗传学研究,包括家系调查和用8种限制性酶及由X染色体Xp21区不同部位获得的9种DNA探针,对Xp21区的13个位点进行了RFLP分析。
