1.Newcastle disease (ND), also called Asian fowl plague or pseud-fowl plague, is an avian acute, highly contacted, contagious disease caused by Newcastle disease virus (NDV). Outbreak of Newcastle disease was first reported in poultry from Java, Indonesia in 1926. The disease currently has a worldwide distribution and causes tremendous loss to poultry industry. It was defined as a list A disease by the Office International des Epizooties (OIE).
新城疫(Newcastle disease，ND)又称亚洲鸡瘟或伪鸡瘟，是由新城疫病毒(Newcastle disease Virus，NDV)引起的一种禽类急性、高度接触性、致死性传染病，自1926年首次暴发于印度尼西亚的爪哇(Java)后，迅速在世界各地传播，给养禽业带来了巨大损失，被世界动物卫生组织(OIE)列为A类传染病。
2.A recombinant Fowl pox virus plasmid pUTAL3CP1 was constructed by inserting the P1 2A structural protein precursor gene and proteinase 3C gene of FMDV into the downstream of hybrid promotor ATI P7 5 and tandem promotor P7 5 of fowl-pox virus expression vector pUTA 16LacZ respectively. The recombinant FPV (rFPV) was obtained by homologous recombination, RT PCR, IFA assay and Western blotting assay.
将口蹄疫病毒 (FMDV)衣壳蛋白前体P1 2A基因和蛋白酶 3C基因分别插入到鸡痘病毒表达载体质粒pUTA 1 6LacZ的复合启动子和单一启动子的下游 ,构建共表达重组鸡痘病毒转移载体质粒pUTAL3CP1 ,与 2 82E4株FPV共转染鸡胚成纤维细胞 ,经RT PCR、IFA及Westernblotting检测 ,成功获得能正确表达目的蛋白的重组鸡痘病毒株vU TAL3CP1。
3.A lot of fowl are like chicken, turkey, guinea fowl, quail, duck, goose to wait to be able to be affected come on, but see more with chicken, turkey, duck and goose, feel most easily with turkey and chicken, incidence of a disease and mortality are very tall; The easy sensibility of the waterfowl such as duck and goose is inferior, but can bear poison or recessive infection, also can have a large number of death sometimes.
许多家禽如鸡、火鸡、珍珠鸡、鹌鹑、鸭、鹅等都可感染发病，但以鸡、火鸡、鸭和鹅多见，以火鸡和鸡最为易感，发病率和死亡率都很高；鸭和鹅等水禽的易感性较低，但可带毒或隐性感染，有时也会有大量死亡。
4.A pair of specific primers were designed and synthesized according to the published sequences of TK gene of ILTV. The DNAs of five strains ILTV were amplificd by two temperature PCR using this pair of primers and ILTV specific 647 base pairs DNA products amplified by this primers were obtained, but not from NDV, IBV, ARV, avian salmonella, fowl mycoplasma and fowl pasteuralla 6 different other avian pathogenic viruses and bacteria.
该对引物对 5株ILTVDNA均扩增出与预期大小相一致的 64 7bp的扩增产物 ,而对新城疫病毒 (NDV )、传染性支气管炎病毒 (IBV)、禽呼肠孤病毒 (ARV)、禽沙门氏菌、鸡毒霉形体和禽巴氏杆菌等 6种禽病病原体的扩增 ,结果全部为阴性。
5.8. There were length differences when using the same primer to amplify the mtDNA HVS-1 of black normal feather Wugu chickens and Guinea fowl. The PCR production sequence length of them were 539bp and 498bp respectively, the same region sequence of mule chicken were the same as Guinea fowl or black normal feather Wugu chickens.
8、黑羽常羽乌骨鸡和珍珠鸡的线粒体D环高变区序列存在长度变异，乌骨鸡的此段序列长度为539hp，珍珠鸡的序列是498hp，而骡鸡个体的此段序列或同于乌骨鸡、或同于珍珠鸡。
6.Both contents of Carnosine from Black-bone silky fowl and White plymouth rock were determined by HPLC,the results showed that the content of Carnosine was much higher in the muscles of Black-bone silky fowl than that in White plymouth rock with 0.45% versus 0.22%.
结果表明:该法适合于肌肉中肌肽的测定,测定结果显示,乌骨鸡肌肉中肌肽量约0.45%,普通白洛克鸡肌肉中约0.22%。
7.SE Asian jungle fowl considered ancestral to domestic fowl.
东南亚原鸡，被认为是家禽的祖先。
8.A lot of fowl are like chicken, turkey, guinea fowl, quail, duck, goose to wait to be able to be affected come on, but see more with chicken, turkey, duck and goose, feel most easily with turkey and chicken, incidence of a disease and mortality are very tall; The easy sensibility of the waterfowl such as duck and goose is inferior, but can bear poison or recessive infection, also can cause large quantities of death sometimes.
许多家禽如鸡、火鸡、珍珠鸡、鹌鹑、鸭、鹅等都可感染发病，但以鸡、火鸡、鸭和鹅多见，以火鸡和鸡最为易感，发病率和死亡率都很高；鸭和鹅等水禽的易感性较低，但可带毒或隐性感染，有时也会造成大批死亡。
9.Clinical features of Sick fowl are listlessness, inappetence, eye closure and sopor, mouth breathing, cough, grunting, swollen face, lachrymation,cockscomb lean; laying rate drop, eggshell achromachia;Kelly and yellow-white viscous loose stool or white water-like diarrhea;Skin-type, mucomembranous fowl pox aroused by poxvirus.
病禽临床表现为精神萎糜、食欲不振、闭目昏睡、张口呼吸、咳嗽、呼噜、肿脸、流泪、鸡冠侧倒；产蛋率下降，蛋壳发白；排黄绿色、黄白色粘稠样稀粪或白色水样腹泻；以及痘病毒引起的皮肤型，粘膜型鸡痘。
10.Genetic diversity of red jungle fowl in China (Gallus gallus spadiceus) and red jungle fowl in Thailand (Gallus gallus gallus) was evaluated with 29 microstaellite loci, the genetic variability within subspecies and genetic differentiation between subspecies were estimated. The results showed that the 168 alleles were amplified with the number of alleles per locus from 2 to 13. The average expected heterozygosity and polymorphism information content (PIC) of all loci were 0.5780 and 0.53, respectively.
利用29个微卫星DNA标记对来自中国的红原鸡Gallus gallus spadiceus亚种和来自泰国的红原鸡Gallus gallus gallus亚种进行遗传多样性分析,评估亚种内的遗传变异和亚种间的遗传分化,结果表明:共检测到168个等位基因,每个位点的等位基因数从2到13不等,所有位点平均的期望杂合度和PIC值分别为0.5780和0.53。
