1.Effect of epidermal growth factor on cultured rat hepatocytes poisoned by CCl 4
Effect of epidermal growth factor on cultured rat hepatocytes poisoned by CCl~4
2.Notes Light and electron microscopic study on Lymphocystis of cage cultured kelp bass, Epinethelus moara (Temminck et Schlegel)
Notes Light and electron microscopic study on Lymphocystis of cage cultured Kelp bass，Epinethelus moara（Temminck et Schlegel）
3.EFFECTS OF SALVIA MILTIORRHIZA BUNGE (SMB) ON LIPID PEROXIDATION OF CULTURED HUMAN FATAL HEPATOCYTES
EFFECTS OF SALVIA MILTIORRHIZA BUNGE （SMB） ON LIPID PEROXIDATION OF CULTURED HUMAN FATAL HEPATOCYTES
4.Here we show that direct elevation of cytoplasmic Ca~_(2+) by extracelluar application of a low concentration of ryanodine, which activated Ca~_(2+) release from intracellular stores, up-regulated Cdc42/Rac, but down-regulated RhoA, in cultured cerebellar granule cells and HEK293T cells.
利用生物化学、钙成像和神经元生长锥转向分析技术,我们发现在转染了野生型Rho GTPases 的人胚胎肾细胞系(Human embryonic kidney 293T cells,HEK293T cells)和原代培养的小脑颗粒细胞(Cultured cerebellar granule cells)中,直接升高细胞内的钙离子浓度可以升高Cdc42/Rac 的活性,同时降低RhoA 的活性。
5.OxLDL-induced Cytotoxicity and Its Inhibition by API_(0134) in Cultured Porcine Aortic Endothelial Cells
OxLDL－induced Cytotoxicity and Its Inhibition by API_（0134） in Cultured Porcine Aortic Endothelial Cells
6.Methods The cells obtained from human lipoaspirates were plated on culture dishes coated with human fibronectin and were cultured in DMEM containing 2% FBS. Cells of passage 2 cultured in EGM-2 (2% FBS) served as the induced cells (experimental group), with cells cultured in DMEM (2% FBS) as the non-induced cells (control group).
方法脂肪抽吸术获取人体脂肪组织,消化法得到的细胞接种在Fibronectin包被的培养皿内,用含2%胎牛血清的DMEM培养,传至第2代,分诱导组(EGM-2,2%FBS,VEGF,bFGF等)和非诱导组(DMEM,2%FBS)两组进行培养。
7.Results Most of the peripheral blood EPCs and MNCs were proliferated adhesively after being cultured for 3 days. Spindle cells were significantly increased after being cultured for 6 days. After 12 days all cultured cells were harvested for identification of EPCs markers.
结果外周血内皮祖细胞和单个核细胞共同培养3d大部分贴壁密集生长,培养6d梭形细胞增多,培养12d收获细胞进行鉴定。
8.Methods: DPM cells were cultured up to 35 days in two groups. The control group was cultured in DMEM supplemented with 15% FCS,50 μg/ml ascorbic acid and 10 mmol/L β-glycerophosphate. The cells in experimental group were cultured in above mentionned medium containing 33.3 nmol/L of PTH.
方法 :细胞在对照组 (含 φ =15 %FCS、10mmol/Lβ 磷酸甘油和 5 0 μg/ml抗坏血酸的DMEM培养液 )和实验组 (含33.3nmol/LPTH的对照组培养液 )连续培养 35d ,每 3～ 4d换液一次。
9.Results Cultured for 72 hours , the morphous of cell of the experimental group grew clustering and began to change from round to irregularity, appearing rough cell face and barb pustute. The longer cell cultured, the more obvious the dendritic structure is. The experimental group cell cultured for 12 days had the most typical dendritic structure.
结果在培养的第72h后实验组和阳性对照组细胞形态开始变化,随着培养时间的延长,树突状结构更加明显,第12天细胞呈典型的树突状细胞形态;
10.Methods Human PBMC were co cultured with porcine vascular endothelial cell line PIEC for 18h. The mixed un co cultured PBMC and PIEC were used as controls.
方法 人外周血单个核细胞 (PBMC)与猪内皮细胞系 PIEC共培养 18h,对照组为未共培养的 PBMC和 PIEC。
