1.Transcription factors ABI3 (abscisic acid-insensitive 3), LEC2 (leafy cotyledon 2) and FUS3 (fusca3) interact with RY element and controls many events during seed development.
RY元件控制某些基因的种子特异性表达,与RY元件相互作用的是ABI3(abscisic acid-insensitive3)、LEC2(leafy,cotyledon2)、FUS3(fusca3)类转录因子。
2.Kanamycin can stronglyinhibited the induction of cotyledon of non-heading chinese cabbage; 100% of explantsturned white on the selection medium when adding Kanamycin up to 15mg/i. Notinhibiting the inductive rate of cotyledon petioles, Carbeniciilin markedly shorten theinduction time of adventitious buds ,from control 20 days to current 14 days.
卡那霉素对不结球白菜子叶的分化有强烈的抑制作用,Km为15mg/L时产生100%白化外植体:羧苄青霉素不仅不抑制带柄子叶的不定芽分化,反而可使不定芽分化的时间由20天提前至14天;
3.2) Study of stress reaction to injured soybean cotyledon:The changes of UBC were observed with high sensitive single photon counter device in the stress reaction experiments of the soybean cotyledon (etilolation and green) after being injured.
2.用单光子计数系统观测萌发的大豆黄化苗及绿苗子叶损伤后的应激反应变化。
4.A hign efficient in vitro regeneration from cotyledon explant of chilli pepper was described in this report,including two conventional varieties,Bulgaria pointed pepper and Chiyan 6.The effect of genotyge,hormone,seeding age,organic nutrient component and AgNO-3 et al on the tissue culture from pepper cotyledon explants was investigated.
以辣椒常规品种保加利亚尖椒与赤研6号为材料,探讨了激素、苗龄、有机成分、AgNO3等因素对子叶再生的影响.
5.1. Research on genetic transformation methods of soybean(1) Research on transformation of salt tolerance gene into cotyledon node of soybean by Agrobacterium tumefaciens-mediated MethodThe cotyledon nodes of Jinong13 and Jinong17 were chosen as experimental materials to transform salt tolerance genes BADH and CMO by using Agrobacterium tumefaciens-mediated Method.
结果表明,浓度(OD值)在0.5-0.6左右,侵染25min时的子叶节的侵染效果最好,并采用NaCl浓度为200mmol/L的含盐培养基对其进行筛选,同时将转化的子叶节进行分化出苗后进行PCR检测和Southern杂交,证明外源目的基因胆碱单加氧酶基因(CMO)和甜菜碱醛脱氢酶基因(BADH)已分别整合到大豆基因组中。
6.The optimum concentration to induce cotyledon and callus of cotyledon for formation of adventitious root was 1.0mg/l,higher or lower concentration was unsatisfactory effect.
结果表明,诱导子叶和子叶的愈伤组织形成不定根的最适浓度为1.0mg/l,过高或过低效果都不令人满意。
7.The study has developed an efficient and fast method for shoot differentiation from cotyledon explant of colour cayenne pepper.
选用辣椒(CapsicumannuumL )杂交F1代2个彩色品种(橙色和紫色)的子叶进行诱导不定芽分化、生长及生根试验,建立了2个彩色辣椒品种的快速繁殖植株再生体系。
8.The report studied the effects of IOA and R-6G on mitochondria inactivation of cotyledon protoplast of non-heading Chinese cabbage 91 HQiu -21 (Aijiaohuang).
以 91H秋 - 2 1(矮脚黄 )不结球白菜为试材 ,研究了碘乙酰胺 (IOA)和罗丹明 (R 6G)对子叶原生质体线粒体失活效果的影响。
9.The content of total proteins (TP) in the mature cotyledon of red lotus (Nelumbo nucifera Gaertn)reached 24. 35g/100 g dry sample: the accumulation pattern of its stoop proteins (SP) was similar to that ofLeguminosae seeds, i. e. with increasing maturity, the content of SP reached abruptly to more than 86% of theTP content.
红莲(NelumbonuciferaGaertn)成熟于叶总蛋白含量达24.35g/100g干样品,其贮存蛋白(SP)的积累模式与豆科种子相似,即随成熟度的提高,SP猛增至占总蛋白含量的86%以上。
10.The resistance to downy mildew [Pseudoperonospor cubensis(Berk.et Curt.) Rostov. ] of cucumber can be induced when the cucumber seedlings are treated by the copolymer of chlorine and potassium ions with 2g/kg,5g/kg,(10g/kg),15g/kg and 20g/kg concentration at the stages of cotyledon and first true leaf.
在黄瓜幼苗子叶期及第一真叶期用浓度为2g/kg、5g/kg、10g/kg、15g/kg、20g/kg的氯钾离子共体液进行诱导处理,可使黄瓜植株产生对霜霉病[Pseudoperonospor cubensis(Berk.et Curt.)