1.The cell survival rate was drastically different between BEL 7402 Sep15 cell and both BEL 7402 pcDNA cell and BEL 7402 Sep15 cell after foreign H2O2 reactive oxygen stress (P< 0 05).
检测不同浓度外源性H2O2氧应激处理后3种细胞BEL-7402-Sep15、BEL-7402-pcDNA3.1和BEL-7402的细胞存活率,结果显示BEL-7402-Sep15的细胞存活率明显高于BEL-7402-pcDNA3.1和BEL-7402(P
2.Objective To comparatively study the apoptosis-inducing effects of chemotherapy and thermochemotherapy of ADM on human hepatocellular carcinoma cell line(BEL7402) and human multidrug-resistant hepatocellular carcinoma cell line(BEL7402/ADM).
目的探讨阿霉素(ADM)加热化疗对人肝癌细胞BEL7402(以下简称BEL7402细胞)及其耐药株BEL7402/ADM细胞(以下简称BEL7402/ADM细胞)的凋亡诱导作用。
3.Objective: To investigate the inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris (STT) on liver cancer cell line BEL-7402. Method: MTT, SRB, Wright staining, acridine orange staining, flow cytometry, and Immunofluorescence microscopy were used to evaluate the effects of STT on BEL-7402 cell line.
目的 :探讨蒺藜皂苷 (STT)对体外培养的人肝癌细胞BEL 74 0 2的增殖抑制作用及诱导凋亡作用。 方法 :应用MTT与SRB法检测蒺藜皂苷对人肝癌细胞BEL 74 0 2的抑制作用 ,采用形态学观察以及流式细胞术 (FCM)检测了STT诱导人肝癌细胞BEL 74 0 2凋亡的效应 ,采用流式细胞术检测BEL 74 0 2细胞Bcl 2蛋白表达。
4.Objective: To investigate the expression of histone deacetyase4 (HDAC4) in human liver carcinoma cell line Bel-7402 and to explore the regulatory effects of HDAC4 on the proliferation and differentiation of Bel-7402. Methods: Carcinoma cells Bel-7402 was treated with different concentrations of sodium phenylbutyrate (SPB), an inhibitor of HDAC4. Expression of HDAC4 mRNA in Bel-7402 cells was analyzed by RT-PCR before and after SPB treatment.
目的:研究组蛋白去乙酰化酶4(histone deacetyase4,HDAC4)在人肝癌细胞株Bel-7402中的表达,及其对Bel-7402细胞增殖和分化的调控作用。
5.So, we first studied the innate resistant mechanism of HCC Bel7402 cell line, and then we successfully established a 161 fold resistant cell line named Bel5-FU20000 in vitro by continuous stepwise exposure of Bel7402 cells to 5-FU for 6 months.
为此，我们首先研究了人肝癌Bel_(7402)细胞株的天然耐药性及其机制，继而又以Bel_(7402)作为亲本细胞，经过体外约6个月的多步骤连续5-FU筛选，得到了对5-FU耐药约161倍的5-FU耐药细胞系Bel_(5-FU20000)，并对其生物学特征及耐药机理进行了研究。
6.The expression of P glycopro tein in BEL 7402 and BEL 7402/ADM cells was determined by using cell ELASA method. The cytotoxicity of drugs was determined by using MTT assay, and the intracellular concentration of doxorubicin in BEL 7402/ADM was determined using a spectrofluorometer.
方法 将BEL 740 2细胞连续培养在含阿霉素 (ADM )的培养液中诱导耐药细胞株BEL 740 2 /ADM ,用cell ELASA法检测细胞膜表面P gp的表达 ,细胞毒试验采用MTT法 ,用荧光分光光度法测定细胞内ADM浓度。
7.The BEL 7402 Sep15 cell line, which expressed the high levels of Sep15 by transfecting the cultured hepatocarcinoma cell line BEL 7402 with pcDNA3.1 Sep15 was generated. From morphologic investigation, cell growth curve, clone formation and nude mice tumor growth curve, the relationship between Sep15 and hepatocarcinoma cell line BEL 7402 was determined. Furthermore, the redox reaction of sep15 was detected by MTT assay.
方法:应用RT-PCR方法获得人Sep15的全长cDNA基因,并重组到真核表达载体pcDNA3.1(+)上,将重组质粒pcDNA3.1-Sep15转染到人肝癌细胞株BEL-7402,得到Sep15高表达的细胞株BEL-7402-Sep15,然后通过一系列体内和体外实验研究Sep15与肝癌细胞的关系及其抗氧化作用。
8.CONCLUSION Decrease of apoptosis may be related to multidrug resistance of human hepatocellular carcinoma cells Bel7402/5-FU. The up-regulation of anti-apoptotic genes and down-regulation of pro-apoptotic genes are involved in the development of multidrug resistance in Bel7402/5-FU cells.
结论 人肝癌Bel74 0 2 / 5 FU耐药株的多药耐药性可能和凋亡减少有关 ,抗凋亡因子bcl xl表达上调 ,促凋亡因子 p5 3和cpp32表达下调可能参与Bel74 0 2 / 5 FU耐药株的多药耐药。
9.The statistical analysis was performed using one-way ANOVA and student t test. Results: SPB significantly decreased the expression of HDAC4 in Bel-7402(0.88±0.13) vs (0.12±0.04), P
结果:SPB处理后Bel-7402中HDAC4 mRNA的表达水平降低(0.88±0.13,0.12±0.04,P
10.Results: The CLT efficiency of the three cell lines of BEL-7402,QBC,and BXPC-3 were 26.99%,2.25% and 30.36% repectively. The CLT efficiency of QBC was significantly lower than those of BEL-7402 and BXPC3(P
结果:BEL-7402,QBC,BXPC3 3系细胞阳离子脂质体介导的瞬时转染率分别为26.99%、2.25%和30.36%,BEL-7402和BXPC-3同QBC细胞有显著差异(P
