1.Objective To investigate the experiment conditions of albumin cobalt binding assay (ACB assay). And to evaluate the capacity of human albumin to bind cobalt in patients with myocardial ischemia.
目的 探讨白蛋白-钴结合实验(Albumin cobalt binding assay, ACB assay)检测缺血修饰白蛋白(Ischemia modified albumin,IMA)的实验条件,初步评价心肌缺血病人的白蛋白-钴结合能力。
2.Methods We stimulate HT29 human colon cancer cell with different concentration of hydrogen peroxide or the same concentration of hydrogen peroxide in different time, through which mimicking the oxygen stress environment in the colon lumen, then confirm the transcription activity of VEGF gene with Run-on assay, Northern Blot, ELISA assay, and further, analyze the correlation between the transcription activity of VEGF gene with the strength of stimuli or the time course.
方法使用H2O2模拟大肠腔内氧化应激环境,通过不同浓度H2O2或相同浓度H2O2不同时间刺激人结肠癌细胞HT29,使癌细胞处于氧化应激状态,应用核连缀分析(Run-on assay)、northern blot、ELISA来观察VEGF基因转录活性,RNA以及蛋白质的表达水平,并进一步分析刺激强度、刺激时间与VEGF基因转录活性的关系。
3.[Methods] The models of DNA damage of ccc-HPF-1 cells were established by treatment of the cells with B(a)P plus S9 at 10 to 100 μmol/L for 3 hours with or without 24 hours recovery for DNA repairing. The DNA damage was detected by single cell gel electrophoresis assay, SCGE (comet assay).
[方法] 建立ccc-HPF-1细胞DNA损伤模型,在S9活化状态下分别以10-100μmol/L B(a)P处理细胞3h收获细胞或再恢复24h,用碱性单细胞凝胶电泳(comet assay)鉴定DNA损伤和修复情况,免疫印迹(Western blot)方法检测JWA和hsp70的表达水平。
4.This paper described an assay for detection of AFP with Immune Enzyme Dot Assay (IEDA) on nitrocellulose paper (NCP) . The principle and the process of this method were stmilar to those of ELISA-sandwich method.
本文以硝酸纤维素膜(Nitrocellulose paper,NCP)作载体首次建立了检测AFP的免疫酶斑点试验(Immnue Enzyme Dot Assay,IEDA),其原理与ELISA夹心法基本相同。
6.The present study was undertaken to evaluate the levels of MIF in plasma and PF of women with EM by enzyme linked immune sorbent assay (ELISA) , to examine the correlation between MIF levels and the stage of the disease and the phase of menstrual cycle, and to determin the role of MIF in promoting the disease.
本研究用酶联免疫吸附法(enzyme linked immune sorbent assay，ELISA)第一次同时测定了EM患者血浆和PF中MIF的水平，并分析MIF水平与疾病的分期和月经周期之间的关系，探讨MIF在EM发病过程中的作用。
7.Enzyme-linked immunosorbent assay (ELISA) was developed for analysing oxytetrocycline(OTC) from the bee products using the anti-OTC antibody.
本研究通过制备土霉素(oxytetracycline, OTC)的特异性抗体,建立了蜂产品中土霉素残留的酶联免疫吸附分析法(enzyme-linked immunosorbent assay, ELISA)。 具体包括以下四个方面的内容:
8.We have measured the content of human plasma glutathione in 40 normal subjects, 20 untreated patients with malignant tumors and 20 patients under treatment with CDDP, CTX and ADR chemotherapy by glutathione reductase recycling assay.
本文用谷胱甘肽还原酶循环法(Glutathione Reductase Recycling Assay)测定40例正常人,20例化疗前恶性肿瘤病人及20例以顺铂、环磷酰胺及阿霉素为主的化疗病人的血浆谷胱甘肽含量.
9.Nondestructive Assay (NDA) techniques have become powerful tools for quantitative determination of special nuclear materials (SNM), and played an important role in nuclear material control and accounting (MC&A), also in inspection of Nuclear Safeguards.
无损分析(NDA：Nondestructive assay)技术已经成为定量分析特种核材料(SNM：special nuclear materials)的重要工具，它在核材料控制与衡算(MC&A)方面以及在核保障核查工作中起着重要作用。
10.Tomographic gamma scanning (TGS) method is one of the most advanced non-destructive assay (NDA) methods.
层析γ扫描(Tomographic Gamma Scanner-TGS)技术是无损分析(Non-Destructive Assay-NDA)技术中最先进的分析技术之一。
