1.Pancreatic Amylase, P-AMY is a kind of α-Amylase. It can catalyze the hydrolysis of α-1, 4 polymeric glucose and play a key role in the degradation of starch and liver sugar in high animals, plants and microorganism.
胰α-淀粉酶(Pancreatic Amylase,P-AMY)是一类α-淀粉酶(Amylase,AMY)同工酶,催化α-1,4-葡萄糖多聚体的水解,并对高等动植物以及微小生物有机体中淀粉和肝糖的降解起重要作用。
2.Four isozymes including esterase (EST), α amylase, cytochrome (COD) and peroxidase (POD) of roots and leaves at vegetative stage, and of stem leaves and flower buds at reproductive stage were compared in six F 1 and their parent lines.
以萝卜3个不育系A1、A5、A6与2个自交系54、55号配组的6个F1代及亲本为材料,分别在营养生长期取根、叶,生殖生长期取茎生叶、花蕾,对酯酶(EST)、α-淀粉酶(α-Amylase)、细胞色素氧化酶(COD)、过氧化物酶(POD)进行比较研究。
3.To understand the expression of PSP and the relationship with α -Amylase (AMY), we research the experession of these two genes in different postnatal stages in mouse and pig by RT-PCR and Q-PCR (fluorescence quantitative Polymerase Chain React).
为了弄清PSP的表达情况,以及它与α-AMY(α-Amylase)表达的关系,本研究根据已知的cDNA序列设计引物,利用RT-PCR、荧光定量PCR(fluorescencequantitative Polymerase Chain React,Q-PCR)对猪及小鼠的PSP、AMY在腮腺生后发育不同时期的表达情况进行了研究。
4.The results showed that its yield was inversely proportional to the concentration of α-amylase and reaction time when the maize starch was hydrolyzed by α-amylase; the granular maize starch was converted into hollow-porous starch by ct-amylase, furthermore it was cracked to the fragments, it was discovered that there were laminated growth ring structure inside starch granular shown on such cracked granular fragments; The crystallinity of maize starch granule can not he increased by the enzyme hydrolysis; the thermodynamic stability of the product was reduced when the hydrolysis time was long.
检测结果表明,α-淀粉酶催化水解玉米淀粉时,其收率与酶的浓度和反应时间成反比;颗粒状玉米淀粉发生酶催化水解时,α-淀粉酶首先使淀粉形成多孔状结构,并进一步使颗粒破裂,断裂的颗粒碎片上显示出淀粉颗粒内部具有层状的生长环结构;酶催化水解不能提高玉米淀粉颗粒的结晶度;酶解时间较长时,产品的热稳定性降低。
5.The β-amylase activity in Shang-24 was higher than that in Cabernet Sauvignon, which was caused by two reasons. One was that the Km of β-amylase was lower in Sahng-24 berries, which led to the higher affinity of β-amylase with substance;
毛葡萄商-24果实β-淀粉酶活性比赤霞珠高的原因为:其β-淀粉酶Km值小,表明其对底物的亲和力大于赤霞珠;
6.A acid-resistance α-amylase producing strain-ASA-1 was screened from vinegar grains, which was identified as candida aquatica belonging to candida genus. ASA-1 was used as startup strain to produce ASA-UD86 strain of higher amylase producing capability by UV mutation and DES complex mutation, the survival rate of amylase reached to 146 u/g. (Tran. by YUE Yang)
从醋醅中筛选得到一株产耐酸性α-淀粉酶的野生菌ASA-1,经初步鉴定为假丝酵母属的水生假丝酵母(Candidaaquatica),以ASA-1为出发菌株,通过紫外诱变(UV)和硫酸二乙酯(DES)复合诱变,获得产酶较高的ASA-UD86菌株,酶活达到146u/g。
7.Activities of αa-,β-amylase and P-, R-enzyme were assayed comparatively. Two of them, P-cnzyme and α-amylase, were found to be deficient in the sterile anthers, i. e., 0,3 μg Pi/spl·h for P-enzyme and 1.2 IDC/spl · h for α-amylase.
对比分析各株系发育中花药的α-,β-淀粉酶,P-酶,R酶活力,得出P-酶活力为0.3μgPi/spl·h,α-淀粉酶活力为1.2 IDC/spl·h.
8.the activity of amylase (α-amylase and β-amylase)、soluble acid invertase (SAI)、sucrose phosphate synthase (SPS)、sucrose synthase (SS) and phenylalanine ammonia lyase (PAL) in the berries of Shang-24 and Cabernet Sauvignon also increased more remarkably comparing with those before veraison.
淀粉酶(α-淀粉酶和β-淀粉酶)、可溶性酸性转化酶(SAI)、蔗糖磷酸合酶(SPS)、蔗糖合酶(SS,分解方向和合成方向),苯丙氨酸解氨酶(PAL)均明显升高。
9.Chemical and biological methods were employed to determine several digestive enzymes including pepsin,tryptase,amylase,cellulase and lipase and isozymes including esterase(EST),malic dehydrogenase(MDH),glutamate dehydrogenase(GDH),alkaline phosphatase(ALP),lactate dehydrogenase(LDH) and amylase(AMY) in mature ova and during embryonic development in prawn Macrobrachium rosenbergii.
采用生物化学方法分别测定了罗氏沼虾胚胎发育期5种消化酶:胃蛋白酶、类胰蛋白酶、淀粉酶、纤维素酶和脂肪酶的比活力,测定了6种同工酶:酯酶(EST)、苹果酸脱氢酶(MDH)、谷氨酸脱氢酶(GDH)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)和淀粉酶(AMY)在成熟卵细胞和胚胎发育时期的表达特征。
10.Results All bacteria we investigated could produce varying level amylase, the highest activity of amylase was from the Bacillus Cereus.
结果地衣芽胞杆菌、枯草芽胞杆菌、蜡样芽胞杆菌和纳豆芽胞杆菌都能产生淀粉酶,以蜡样芽胞杆菌产生的淀粉酶较多。