1.Method:The samples were separated on a Luna C18 column (4.6 mm×250 mm, 5μn) with the mobile phase of acetonitrile -0.1% phosphoric acid(28:72). The flow rate was 1. 0 mL·in-1, and the detection wavelength was set at 285 nm.
方法:采用反相高效液相色谱法,以Luna C18(4.6mm×250mm,5 μm)为色谱柱,乙腈-0.1％磷酸(28:72)为流动相,检测波长285nm,流速1mL·min-1,柱温30℃。
2.The chromatographic column is LUNA 5μm C 18 (2)(150×4.60mm,U.S.A),the mobile phase is 2.5mmol·L -1 phosphoric acid-acetonitrile (1∶1),and wavelength is 220nm.
色谱柱为LUNA 5 μmC18(2 ) (15 0×4 .6 0mm ,U .S .A) ,流动相为 2 .5mmol·L-1磷酸溶液 -乙腈 (1∶1) ,检测波长 2 2 0nm。
3.Subsequently, the bitter fractions recognized were separated by reversed phase HPLC on LUNA C18 column and confirmed by sensory testing, three peptides with the most bitter flavor(p2, p4, p8) were obtained.
该苦味部分经LUNA C18反相色谱进一步纯化并经感官鉴评，获得三个苦感最强的苦味肽(p2，p4，p8)；
4.METHODS A Luna 5 u C 18 (2) column was used. The mobile phase consisted of methanol-0.1% phosphoric acid solution (85∶15). The detected wavelength was set at 254 nm.
方法 使用Luna 5uC18(2 )柱 (15 0mm× 4.6mm ,ID) ,以甲醇 - 0 .1%磷酸溶液 (85∶15 )为流动相 ,检测波长为 2 5 4nm。
5.(6). The characteristics of the microwave radiation of the lunar regolith was discussed on the base of the detail research into the complex permittivity of lunar materials. The penetration depths of microwave radiation of the four channels of the radiometer at Apollo and Luna landing sites were estimated.
(6) 在对月球物质的复介电常数进行详细研究的基础上,探讨了月壤的微波辐射特性,并估算了Apollo和Luna登月点的微波辐射在月壤中的穿透深度。
6.Methods HPLC method was performed on Luna C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile- water (30∶70) and the UV detective wavelength was set at 270 nm.
方法采用高效液相色谱法,色谱柱为Luna C18(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70),检测波长270nm。
7.Methods Phenomenex Luna NH2(5μm,250mm×4.6mm ) unlalytical column was used;
方法采用Phenomenex Luna氨基键合柱(5μm,250 mm×4.6 mm);
8.Separation was carried out on the column of Phenomenex Luna NH_2(4.6mm×250mm,5μm)at 30℃.
色谱柱为Phenomenex Luna NH_2(4．6 mm×250 mm,5μm)；
9.The plasmids pUbiPina, pUbiPinb and pCaI-Neo containing Pina, Pinb and Neo genes were introduced into four donor durum wheats of Luna, Ofanto, Venusia and Latino×Lira through cotransformation with the optimal condition by particle bombardment. The results of tissue culture showed that the scutella bombarded from four durum wheat cultivars (line) had the high embryogenesis frequencies of 99.4%, 100.0%, 95.2% and 97.0% respectively.
采用优化后的轰击方案,以4种硬粒小麦Luna、Ofanto、Venusia和Latino×Lira的幼胚盾片为外植体,将携带有外源目的基因Pina、Pinb和选择基因Neo的质粒pUbiPina、 pUbiPinb和pCaI-Neo共转化到受体材料中,统计结果显示轰击后的4种外植体材料的愈伤组织诱导率分别为99.4%、100.0%、95.2%和97.0%。
10.A Luna Silica(2) column was used with hexane-dichloromethane-methanol-strong ammonia solution(45∶40∶5∶0.35) as mobile phase.
用Luna Silica(2)色谱柱,以正己烷-二氯甲烷-甲醇-浓氨试液(45∶40∶5∶0.35)为流动相,流速为1.0ml.
