1.Animals were sacrificed on the day 2,5,7,14,and liver grafts were collected for measuring the expression of IDO mRNA,IDO protein and IDO positive cells in the grafts by using of RT-PCR,Western-blot and anti-IDO monoclonal antibody immunochemistry methods respectively.
分别于术后第2、5、7、14d处死受者,取出移植肝,采用逆转录聚合酶链反应(RT-PCR)、Western印迹法及免疫组化法,检测移植肝组织中IDO mRNA表达I、DO蛋白的水平以及IDO在移植肝细胞中的表达。
2.Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
结果 从活化的人白细胞中克隆到IDO基因编码区全长 ,并构建了IDO EGFP融合蛋白表达载体。
3.the serum made from herbal medicine improve the expression of IDO protein and IDO activity, and resume the balance of Th cytokine.
中药血清可提高IDO蛋白质表达及活性,恢复Th细胞因子平衡。
4.Results: Real-time PCR revealed an apparent increase expression of IDO and FasL mRNA in the first 6 hs in KC pretreated with IFN- γ , Catabolic
表达IDO的KC能明显抑制同种异体T淋巴细胞的增殖，但1-MT和抗FasL抗体能部分阻断其增殖抑制作用，并且存在剂量依赖关系。 表达IDO和FasL的KC能阻止同种异体T淋巴细胞于G1中期，诱导其凋亡。
5.Objective To detect the expression of indoleamine 2,3-dioxygenase(IDO) gene in the rat liver grafts after liver transplantation,and discuss the relationship between IDO gene expression and posttransplantation immune rejection.
目的检测吲哚胺2,3双加氧酶(IDO)基因在大鼠肝移植后移植肝中的表达情况,探讨其与急性排斥反应的关系。
6.Over expression of indoleamine 2,3-dioxygenase(IDO)is an important mechanism that is responsible for immune tolerance of tumor.
吲哚胺2,3-二加氧酶(idoleamine2,3-dioxy-genase,IDO)的高表达是导致肿瘤免疫耐受的一个重要机制。 诱导IDO的表达具有两种信号传导途径。
7.Role of ILT and IDO in Dendritic Cells in Inducing Immune Tolerance
ILT及IDO在树突状细胞介导免疫耐受中的作用及机制
8.γ-interferon-induced IDO expression of mesenchymal stem cells in vitro and the immuno-regulatory mechanism
γ干扰素体外诱导骨髓间充质干细胞的IDO表达及其免疫调节作用
9.IDO enzyme activity in MSCs inhibited T-lymphocytes proliferation of MLR cultures.
MSCs的IDO活性能抑制MLC体系中T淋巴细胞增殖率。
10.In comparison to the inbred group,IDO mRNA expression was increased obviously in the allogenic group and the peak expression occurred on the day 7,and high level kept for over 2 weeks.
同种移植组IDO mRNA的表达明显增强,高峰出现在第7d,持续2周以上。
