1.RESULTS ISOC at 10 μmol·L -1 inhibited CONCLUSION ISOC can inhibit the receptor mediated Ca 2+ influx and Ca 2+ release, but it is not a classical Ca 2+ antagonist.
各实验浓度的ISOC对咖啡因在无钙液中的缩血管作用均无影响。 结论ISOC抑制受体中介的钙释放和钙内流，但不是典型的钙拮抗剂。
2.Methods (1) the isolation technique of rat hepatocellular,(2) whole cell patch clamp techniques were used to investigate the effects of 2-APB on store Operated calcium current (Isoc).
结果在急性分离的大鼠肝细胞记录到Isoc,从其电流电压(IV)曲线可以看出该Isoc的反转电位为-2.5mV左右。 在钳制电位为-100mV时,该Isoc为(-664.52±140.44)pA(n=8);
3.Results(1)2-APB(20,40,60,80 and 100)μmol/L produced a concentration-dependent decrease of Isoc with IC_(50)value of(63.36±9.57)μmol/L(n=8);
结果(1)培养的人肝细胞记录到Isoc,从其电流-电压(Ⅰ～Ⅴ)曲线可以看出该Isoc的反转电位为+3．0 mV左右。 在钳制电位为-100 mV时,该Isoc为(-682．83±171．40)pA(n=8)；
4.Methods The changes of the [Ca 2+ ]i increased by NA and high potassium in the presence of ISOC were determined, using fura-2/AM-loaded cells of the blood vessel smooth muscle of the rabbit.
方法 利用钙荧光指示剂Fura 2 /AM负载的家兔血管平滑肌培养细胞 ,动态地观察在ISOC存在下 ,由NA和高钾所增加的 [Ca2 + ]i的改变 ,分析ISOC对NA和高钾所致钙内流和 /或钙释放的影响。
5.AIM: To study the effects of hepatic ischemia/reperfusion (I/R) injury on hepatocellular viability and store-operated calcium channel currents (Isoc) in freshly isolated rat hepatocytes, and the effects of (2-aminoethoxydiphenyl borane) 2-APB on Isoc of hepatocytes after hepatic ischemia/reperfusion injury in rats.
目的:研究缺血再灌注损伤对大鼠肝细胞活性和钙池操纵的钙通道电流(Isoc)的影响及钙通道阻滞剂(2- aminoethoxydiphenyl borane,2-APB)的拮抗作用.
6.Objective To st,udy the effects of 2-APB on store operated calcium current (Isoc) in isolated rat hepatocytes.
目的探讨钙池操纵的钙通道(SOC)抑制剂(2APB)对大鼠肝细胞SOC电流(Isoc)的影响。 方法急性分离大鼠肝细胞,应用全细胞膜片钳记录技术测定大鼠肝细胞Isoc,并观察2APB对该电流的影响。
7.Objective To study the effects of 2-APB on store operated calcium current(Isoc)in human hepatocytes.
目的探讨钙池操纵钙通道(SOC)抑制剂(2-APB)对人肝细胞SOC电流(Isoc)的影响。
8.Conclusions ISOC inhibited the receptor mediated increasing of [Ca 2+ ] i in the heart cells,and it may be partly relate to its antiarrhythmic effects.
结论 ISOC抑制由受体中介的心肌细胞 [Ca2 + ]i 的增高
9.Conclusion 2-APB had a protective effect on isolated rat hepatocytes,probably though inhibiting Isoc.
结论2APB对急性分离的大鼠肝细胞Isoc具有显著的抑制作用,这对于进一步深入进行肝细胞钙超载的发生机制和防治具有重要意义。
10.Conclusion 2-APB has a protective effect on human hepatocytes,probably though inhibiting Isoc.
结论2-APB对培养的人肝细胞Isoc具有显著的抑制作用。
